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首页> 外文期刊>Functional & integrative genomics >Transcriptome analysis of Brassica juncea var. tumida Tsen responses to Plasmodiophora brassicae primed by the biocontrol strain Zhihengliuella aestuarii
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Transcriptome analysis of Brassica juncea var. tumida Tsen responses to Plasmodiophora brassicae primed by the biocontrol strain Zhihengliuella aestuarii

机译：甘草juncea var的转录组分析。 Tumida tsen对血管带菌株Zhihengliuella aestuarii的血管脂肪酸乳酪乳酪的反应

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摘要

Mustard clubroot, caused by Plasmodiophora brassicae, is a serious disease that affects Brassica juncea var. tumida Tsen, a mustard plant that is the raw material for a traditional fermented food manufactured in Chongqing, China. In our laboratory, we screened the antagonistic bacteria Zhihengliuella aestuarii against P. brassicae. To better understand the biocontrol mechanism, three transcriptome analyses of B. juncea var. tumida Tsen were conducted using Illumina HiSeq 4000, one from B. juncea only inoculated with P. brassicae (P), one inoculated with P. brassica and the biocontrol agent Z. aestuarii at the same time (P + B), and the other was the control (H), in which P. brassicae was replaced by sterile water. A total of 19.94 Gb was generated by Illumina HiSeq sequencing. The sequence data were de novo assembled, and 107,617 unigenes were obtained. In total, 5629 differentially expressed genes between biocontrol-treated (P + B) and infected (P) samples were assigned to 126 KEGG pathways. Using multiple testing corrections, 20 pathways were significantly enriched with Qvalue aecurrency 0.05. The resistance-related genes, involved in the production of pathogenesis-related proteins, pathogen-associated molecular pattern-triggered immunity, and effector-triggered immunity signaling pathways, calcium influx, salicylic acid pathway, reactive oxygen intermediates, and mitogen-activated protein kinase cascades, and cell wall modification, were obtained. The various defense responses induced by the biocontrol strain combatted the P. brassicae infection. The genes and pathways involved in plant resistance were induced by a biocontrol strain. The transcriptome data explained the molecular mechanism of the potential biocontrol strain against P. brassicae. The data will also serve as an important public information platform to study B. juncea var. tumida Tsen and will be useful for breeding mustard plants resistant to P. brassicae.
机译：由Plasmodiophora Brassicae引起的芥末球杆菌是一种严重的疾病，影响芸薹属Juncea VAR。 Tumida Tsen是一种芥末植物，是中国重庆制造的传统发酵食品的原料。在我们的实验室中，我们将拮抗细菌Zhihengliuella Aestuarii筛选为P. Brassicae。为了更好地理解生物控制机制，B. Juncea VAR的三种转录组分析。用Illumina Hiseq 4000进行Tumida Tsen，其中一个来自B. juncea，仅接种于P. Brassicae（P），其中一个接种于P. Brassica和Biocontrol Agent Z.Asestuarii（P + B），另一个是对照（h），其中p. brassicae被无菌水代替。 Illumina Hiseq测序总共产生了19.94 GB。序列数据是De Novo组装，获得了107,611个unigenes。总共，将5629种卵体处理（P + B）和感染（P）样品之间的差异表达基因分配给126 kegg途径。使用多种测试校正，用QValue AE显着富集20个途径。 0.05。抗性相关基因，参与发病性相关蛋白质，病原体相关的分子模式触发的免疫和效应触发的免疫信号通路，钙流入，水杨酸途径，反应性氧中间体和丝裂原激活蛋白激酶获得级联和细胞壁改性。生物防治菌株诱导的各种防御反应调动了P. Brassicae感染。植物抗性所涉及的基因和途径由生物控制菌株诱导。转录组数据解释了对P. Brassicae潜在的生物防治菌株的分子机制。数据还将作为学习B. Juncea Var的重要公共信息平台。 Tumida Tsen并将有助于育种芥末植物抗P. Brassicae。

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