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首页> 外文期刊>European Journal of Plant Pathology >Identification of differentially expressed genes in Brassica juncea var. tumida Tsen following infection by Plasmodiophora brassicae
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Identification of differentially expressed genes in Brassica juncea var. tumida Tsen following infection by Plasmodiophora brassicae

机译：鉴定甘蓝型油菜差异表达基因芸苔疟原虫感染后的Tumida Tsen

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摘要

Mustard clubroot, caused by Plasmodiophora brassicae, is one of the most serious diseases affecting Brassica juncea var. tumida Tsen, a mustard plant which is the raw material of a traditional fermented food manufactured in the Chongqing Municipality, P. R. China. We used suppression subtractive hybridization (SSH) to better understand the interaction between B. juncea var. tumida and P. brassicae, and the complex regulation of resistance mechanisms occurring in B. juncea var. tumida after infection by P. brassicae. A total of 1,842 different gene clones were selected from the forward subtracted library (using diseased roots as tester and healthy roots as driver), and 224 positive spots were identified following cDNA array dot blotting. Elimination of polyA tails and sequences shorter than 100 bp generated 196 high-quality gene sequences with an average length of 332 bp. Bioinformatic analysis showed that these 196 sequences represented 173 unigenes, comprising 14 contigs and 159 singlets. Of these, 146 ESTs (84.4 % of the total) were significantly similar to known sequences in plants, the remaining 23 (13.3 % of the total) were of P. brassicae origin. We used quantitative reverse transcription-PCR to analyze the six genes most likely to be involved in disease resistance or the stress response to evaluate the efficiency of SSH, and the results showed that our library data is reliable. Further study of these genes might be helpful for breeding resistance of mustard plants to P. brassicae.
机译：芥菜根病是由芸苔疟原虫引起的，是影响芥菜型油菜的最严重的疾病之一。 tumida Tsen，一种芥菜植物，是重庆市生产的传统发酵食品的原料。我们使用抑制消减杂交（SSH）来更好地了解芥菜型变种之间的相互作用。根瘤菌和芸苔球菌，以及抗药性机制的复杂调控，发生在芥菜芽胞杆菌中。被芸苔假单胞菌感染后的肿瘤。从正向消减文库中选择了1,842个不同的基因克隆（使用患病的根作为测试者，使用健康的根作为驱动器），并在cDNA阵列点印迹后鉴定了224个阳性斑点。消除polyA尾巴和短于100 bp的序列可产生196个高质量基因序列，平均长度为332 bp。生物信息学分析表明，这196个序列代表173个单基因，包括14个重叠群和159个单峰。其中，146个EST（占总数的84.4％）与植物中的已知序列显着相似，其余23个（占总数的13.3％）来自芸苔科。我们使用定量逆转录-PCR分析了最可能与疾病抗性或应激反应有关的六个基因，以评估SSH的效率，结果表明我们的文库数据是可靠的。这些基因的进一步研究可能有助于芥菜植物对芸苔假单胞菌的抗性育种。

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