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首页> 外文期刊>Functional & integrative genomics >Heterologous expression of Brassica juncea annexin, AnnBj2 confers salt tolerance and ABA insensitivity in transgenic tobacco seedlings
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Heterologous expression of Brassica juncea annexin, AnnBj2 confers salt tolerance and ABA insensitivity in transgenic tobacco seedlings

机译:Brassica Juncea Annexin,Annbj2的异源表达赋予转基因烟草幼苗的耐盐性和ABA不敏感性

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摘要

Annexins are multifunctional proteins with roles in plant development and alleviation of stress tolerance. In the present communication, we report on the effect of heterologous expression of Brassica juncea annexin, AnnBj2 in tobacco. Transgenic tobacco plants expressing AnnBj2 exhibited salt-tolerant and abscisic acid (ABA)-insensitive phenotype at the seedling stage. Biochemical analysis showed that AnnBj2 transgenic plants retained higher chlorophyll and proline content, and lower malondialdehyde (MDA) levels compared to the null line under salt stress. They exhibited better water retention capacity compared to the null segregant (NS) line. AnnBj2 overexpression altered the transcript levels of several stress-related marker genes involved in reactive oxygen species (ROS) scavenging and abiotic stress signaling. Taken together, these results suggest a positive role for AnnBj2 in salt stress response upon heterologous expression in tobacco.
机译:annexins是多功能蛋白质,具有植物开发和减轻应力耐受性的作用。 在目前的沟通中,我们报告了甘蓝型juncea annexin,Annbj2在烟草中的异源表达的影响。 在幼苗阶段,表达annb72的转基因烟草植物在幼苗阶段表现出耐盐性和脱盐酸(ABA)敏感的表型。 生物化学分析表明,与盐胁迫下的含氟线相比,Annbj2转基因植物保留更高的叶绿素和脯氨酸含量,低丙二醛(MDA)水平。 与零偏换(NS)线相比,它们表现出更好的水保持能力。 AnnbJ2过表达改变了参与反应性氧(ROS)清除和非生物应激信号传导的几种应激相关标记基因的转录水平。 在一起,这些结果表明在烟草中异源表达的盐应激反应中的AnnbJ2对盐胁迫反应的积极作用。

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