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A molecular analysis of selected marine fishes from the southwest coast of India for species delineation

机译:来自印度西南海岸的选定海洋鱼类的分子分析为物种描绘

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摘要

In this study, mitochondrial genes 16S and cytochrome C oxidase I (COI) were used as taxonomic markers for the molecular analysis of selected fishes from the southwest coastal waters of India, viz., Sillago sihama (Forsskal, 1775), Mene maculata (Bloch & Schneider, 1801), Seriolina nigrofasciata (Ruppell, 1829), and Minous dempsterae (Eschmeyer, Hallacher & Rama-Rao, 1979). Both the gene fragments showed diagnostic differences and divergence within each taxon. The mean nucleotide diversity (Pi) among all the species was estimated as 0.083. The average transition and transversion ratio was 1.63. The average K2P distance of species within genera was estimated as 13.71 %, whereas it was 20.22 % among species within families. The variation was more among the species within families than among the congeneric individuals. The study on the phylogenetic relationships among the species revealed distinct clusters in concurrence with the taxonomic status of the species. The study also suggests that the mtDNA 16S (rRNA) and COI genes can be used as barcodes for the identification of fish species based on sequence information.
机译:在该研究中,用被印度西南沿海水域的分子分析的线粒体基因16s和细胞色素C氧化酶I(COI)被用作分类标志物,用于来自印度西南沿海水域的分子分析。,Sillago Sihama(Forsskal,1775),Mene Maculata(Bloch & Schneider,1801),血清体尼克罗多西亚(Ruppell,1829年)和Minous Dempsterae(Eschmeyer,Hallacher& Rama-Rao,1979)。基因片段都显示出每个分类群体内的诊断差异和分歧。所有物种中的平均核苷酸多样性(PI)估计为0.083。平均转变和颠转率为1.63。估计属于13.71%的属的平均K2P距离估计为13.71%,而家庭在家庭中的物种是20.22%。在家族中的种类中,比在基础个体中的种类中更多。对物种中系统发育关系的研究显示了与物种的分类地位同时发生的明显簇。该研究还表明,MTDNA 16S(RRNA)和COI基因可以用作条形码,用于基于序列信息鉴定鱼类。

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