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Human Mature Adipocytes Express Albumin and This Expression Is Not Regulated by Inflammation

机译：人成熟脂肪细胞表达白蛋白，这种表达不是通过炎症调节

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Aims. Our group investigated albumin gene expression in human adipocytes, its regulation by inflammation and the possible contribution of adipose tissue to albumin circulating levels. Methods. Both inflamed and healthy subjects provided adipose tissue samples. RT-PCR, Real-Time PCR, and Western Blot analysis on homogenates of adipocytes and pre-adipocytes were performed. In sixty-three healthy subjects and fifty-four micro-inflamed end stage renal disease (ESRD) patients circulating levels of albumin were measured by nephelometry; all subjects were also evaluated for body composition, calculated from bioelectrical measurements and an thropometric data. Results. A clear gene expression of albumin was showed in pre-adipocytes and, for the first time, in mature adipocytes. Albumin gene expression resulted significantly higher in pre-adipocytes than in adipocytes. No significant difference in albumin gene expression was showed between healthy controls and inflamed patients. A significant negative correlation was observed between albumin levels and fat mass in both healthy subjects and inflamed ESRD patients. Conclusions. In the present study we found first time evidence that human adipocytes express albumin. Our results also showed that systemic inflammation does not modulate albumin gene expression. The negative correlation between albumin and fat mass seems to exclude a significant contributing role of adipocyte in plasma albumin. Adipose tissue, once just believed a passive organ storing excess energy, is now considered a real endocrine organ, with an important role in the regulation of homeostatic systems. It secretes various proteins that can be classified into molecules acting on metabolic processes like glucose homeostasis and insulin sensitivity, and into inflammatory molecules like interleukin 6 (IL-6), interleukin ljS, Tumor Necrosis Factor-a, C-reactive protein (C-RP), Leptin, and Adiponectin. The adipose tissue may account for 20-25% of systemic IL-6 circulating levels and seems to be involved in the systemic inflammatory response associated with obesity, insulin resistance, and metabolic syndrome.
机译：目标。我们对人脂肪细胞中的白蛋白基因表达，其通过炎症调节以及脂肪组织对白蛋白循环水平的可能贡献。方法。发炎和健康的受试者都提供了脂肪组织样本。进行RT-PCR，实时PCR和Western印迹分析对脂肪细胞匀浆和预脂肪细胞的匀浆。在六十三个健康受试者和五十四个微发炎的肾病（ESRD）循环水平白蛋白的患者中，通过肾小序测量;还评估了所有受试者的主体组合物，从生物电测量和流孔测量数据计算。结果。在脂肪细胞中，在成熟的脂肪细胞中显示白蛋白的明确基因表达。白蛋白基因表达在预脂肪细胞中显着高于脂肪细胞。在健康对照组和发炎患者之间显示白蛋白基因表达的显着差异。在健康受试者和发炎的ESRD患者中，白蛋白水平和脂肪肿块之间观察到显着的阴性相关性。结论。在本研究中，我们发现人类脂肪细胞表达白蛋白的首次证据。我们的结果还表明，全身炎症不调节白蛋白基因表达。白蛋白和脂肪质量之间的负相关似乎排除了脂肪细胞在血浆白蛋白中的显着贡献作用。脂肪组织，刚刚相信储存过量能量的被动器官，现在被认为是一个真正的内分泌器官，在稳态系统的调节中具有重要作用。它分泌各种蛋白质可以分为作用于代谢过程的分子，如葡萄糖稳态和胰岛素敏感性，以及白细胞介素6（IL-6），白细胞介素LJ，肿瘤坏死因子-A，C-反应蛋白（C- RP），瘦素和脂联素。脂肪组织可能占20-25％的全身IL-6循环水平，似乎参与了与肥胖，胰岛素抵抗和代谢综合征相关的全身炎症反应。

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