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首页> 外文期刊>Free Radical Biology and Medicine: The Official Journal of the Oxygen Society >Redox proteomic profiling of neuroketal-adducted proteins in human brain: Regional vulnerability at middle age increases in the elderly
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Redox proteomic profiling of neuroketal-adducted proteins in human brain: Regional vulnerability at middle age increases in the elderly

机译：人脑中神经急性蛋白质的氧化还原蛋白质组学分析：中年的区域脆弱性在老年人增加

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Protein lipoxidation was assessed in the parietal cortex (PC), frontal cortex (FC), and cingulate gyms (CG) in middle-aged and old-aged individuals with no clinical manifestations of cognitive impairment, in order to increase understanding of regional brain vulnerability to oxidative damage during aging. Twenty-five lipoxidized proteins were identified in all the three regions although with regional specificities, by using redox proteomics to detect target proteins of neuroketals (NKT) adduction. The number of cases with NKT-adducted proteins was higher in old-aged individuals but most oxidized proteins were already present in middle-aged individuals. Differences in vulnerability to oxidation were dependent on the sub-cellular localization, secondary structure, and external exposition of certain amino acids. Lipoxidized proteins included those involved in energy metabolism, cytoskeleton, proteostasis, neurotransmission and O-2/CO2, and heme metabolism. Total NKT and soluble oligomer levels were estimated employing slot-blot, and these were compared between age groups. Oligomers increased with age in PC and FC; NKT significantly increased with age in FC, whereas total NKT and oligomer levels were not modified in CG, thus highlighting differences in brain regional vulnerability with age. Oligomers significantly correlated with NKT levels in the three cortical regions, suggesting that protein NKT adduction parallels soluble oligomer formation. (C) 2016 Elsevier Inc. All rights reserved.
机译：在蛋白质富含蛋白质脂氧化在榫胞型皮质（PC），额叶（FC），并在中年和老年人中施加健身房（CG），没有认知障碍的临床表现，以增加对区域脑脆弱性的理解老化期间氧化损伤。在所有三个区域中鉴定了二十五种偶氮氧化蛋白，尽管通过使用氧化还原蛋白质组学检测神经术靶蛋白（NKT）的靶蛋白。在旧的个体中具有NKT加入蛋白质的病例数量较高，但大多数氧化蛋白已存在于中年个体中。易受氧化脆性的差异依赖于某些氨基酸的亚细胞定位，二级结构和外部阐述。脂氧化蛋白包括参与能量代谢，细胞骨架，蛋白质，神经递血和O-2 / CO2的那些，以及血红素代谢。估计槽印迹估计总NKT和可溶性低聚物水平，并且这些在年龄组之间比较。低聚物随着PC和Fc的年龄而增加;在FC中，NKT随着年龄的增长而显着增加，而CG的总NKT和低聚物水平未被修饰，从而突出脑区域脆弱性差异随着年龄的增长。寡聚体与三个皮质区域中的NKT水平显着相关，表明蛋白质NKT内膜等离子体可溶性低聚物形成。 （c）2016年Elsevier Inc.保留所有权利。

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