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首页> 外文期刊>Microbiological Research >The developmental regulator Pcz1 affects the production of secondary metabolites in the filamentous fungus ce:italic>Penicillium roqueforti/ce:italic>
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The developmental regulator Pcz1 affects the production of secondary metabolites in the filamentous fungus ce:italic>Penicillium roqueforti/ce:italic>

机译：发育调节剂PCZ1影响丝状真菌中的次级代谢物的产生

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摘要

Penicillium roquefortiis used in the production of several kinds of ripened blue-veined cheeses. In addition, this fungus produces interesting secondary metabolites such as roquefortine C, andrastin A and mycophenolic acid. To date, there is scarce information concerning the regulation of the production of these secondary metabolites. Recently, the gene namedpcz1(PenicilliumC6 zinc domain protein 1) was described inP. roqueforti, which encodes for a Zn(II)2Cys6protein that controls growth and developmental processes in this fungus. However, its effect on secondary metabolism is currently unknown. In this work, we have analyzed how the overexpression and down-regulation ofpcz1affect the production of roquefortine C, andrastin A and mycophenolic acid inP. roqueforti. The three metabolites were drastically reduced in thepcz1down-regulated strains. However, whenpcz1was overexpressed, only mycophenolic acid was overproduced while, on the contrary, levels of roquefortine C and andrastin A were diminished. Importantly, these results match the expression pattern of key genes involved in the biosynthesis of these metabolites. Taken together, our results suggest that Pcz1 plays a key role in regulating secondary metabolism in the fungusPenicillium roqueforti.
机译：Penicillium Roquefortiis用于生产几种成熟的蓝色脉奶酪。此外，这种真菌产生了有趣的次级代谢产物，例如Roquefortine C，Andrastin A和霉酚酸。迄今为止，存在关于这些次级代谢产物的制备的稀缺信息。最近，描述了INP的NamedPCZ1（PenicilliumC6锌结构粒蛋白1）。 Roqueforti，其用于控制​​该真菌中的生长和发育过程的Zn（II）2Cys6蛋白。然而，它对次生新陈代谢的影响目前是未知的。在这项工作中，我们分析了如何过表达和下调的PCZ1affect如何生产Roquefortine C，Andrastin A和霉蛋白酸InP。 roqueforti。三种代谢物在ThePCZ1下调调节菌株中大幅减少。然而，当PCZ1Was过表达时，只有霉酚酸过度引起，而相反，罗基摩特C和Andrastin A的水平降低。重要的是，这些结果与这些代谢物生物合成中涉及的关键基因的表达模式匹配。我们的结果表明，PCZ1在真菌植物羊毛中调节次生新陈代谢的关键作用。

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  • 来源
    《Microbiological Research》 |2018年第2018期|共8页
  • 作者

    Juan F. Rojas-Aedo; Carlos Gil-Durán; Alejandra Goity; Inmaculada Vaca; Gloria Levicán; Luis F. Larrondo; Renato Chávez;

  • 作者单位

    Departamento de Biología Facultad de Química y Biología Universidad de Santiago de Chile (USACH);

    Departamento de Biología Facultad de Química y Biología Universidad de Santiago de Chile (USACH);

    Millennium Institute for Integrative Systems and Synthetic Biology (MIISSB) Departamento de Genética Molecular y Microbiología Facultad de Ciencias Biológicas Pontificia Universidad Católica de Chile;

    Departamento de Química Facultad de Ciencias Universidad de Chile;

    Departamento de Biología Facultad de Química y Biología Universidad de Santiago de Chile (USACH);

    Millennium Institute for Integrative Systems and Synthetic Biology (MIISSB) Departamento de Genética Molecular y Microbiología Facultad de Ciencias Biológicas Pontificia Universidad Católica de Chile;

    Departamento de Biología Facultad de Química y Biología Universidad de Santiago de Chile (USACH);

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  • 中图分类 生物科学;
  • 关键词

    Zn(II)2Cys6protein; Pcz1; Secondary metabolism; Penicillium roqueforti;

    机译：Zn（ii）2cys6蛋白;pcz1;次生新陈代谢;青霉羊皮;

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