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首页> 外文期刊>Microbial drug resistance: MDR : Mechanisms, epidemiology, and disease >Plasmid-Mediated Colistin Resistance in Escherichia coli O157:H7 Cattle and Sheep Isolates and Whole-Genome Sequence of a Colistin-Resistant Sorbitol Fermentative Escherichia coli O157:H7
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Plasmid-Mediated Colistin Resistance in Escherichia coli O157:H7 Cattle and Sheep Isolates and Whole-Genome Sequence of a Colistin-Resistant Sorbitol Fermentative Escherichia coli O157:H7

机译：质粒介导的大肠杆菌O157中的菌炎耐药性：H7牛和绵羊分离物和耐菌氏山梨糖醇发酵大肠杆菌O157的全基因组序列：H7

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The aims of this study were to investigate the plasmid-mediated colistin resistance genes (mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5), phenotypic colistin resistance in Escherichia coli O157:H7+/H7? strains isolated from cattle and sheep, and whole-genome sequence (WGS) analysis of colistin-resistant sorbitol fermentative E. coli O157:H7. According to the results, 5 of the 49 isolates were found to harbor mcr-2 and/or mcr-3 genes. Three isolates, including a sorbitol fermentative E. coli O157:H7, were found phenotypically resistant to colistin with a minimum inhibitory concentration value of 128?μg/mL. The genome of sorbitol fermentative E. coli O157:H7 did not show 100% similarity to any of the other genome sequences found in the universal genome database. It has also been determined that this isolate carried 62 different antimicrobial resistance genes. This is the first report of plasmid-mediated mcr-2 and mcr-3 genes carrying E. coli O157:H7 from cattle and sheep isolates and WGS of a colistin-resistant sorbitol fermentative E. coli O157:H7. Findings of this study indicate that cattle and sheep can be an important source of colistin resistance in E. coli O157:H7, and slaughterhouse wastewater might be a significant route for dissemination of the plasmid-mediated colistin genes. Therefore, the use of colistin in veterinary medicine should be restricted to reduce the development of resistance. Also it may be necessary to review the non-sorbitol fermentation-based isolation protocol for not missing the sorbitol fermentative E. coli O157:H7 in epidemiological studies.
机译：本研究的目的是研究质粒介导的菌落抗性基因（MCR-1，MCR-2，MCR-3，MCR-4和MCR-5），在大肠杆菌O157中的表型Colistin抗性抗性：H7 + / H7？从牛和绵羊分离的菌株，以及整个基因组序列（WGS）分析对耐菌氏山梨糖醇发酵大肠杆菌O157：H7。根据结果​​，发现49个分离株中的5个用于涉及MCR-2和/或MCR-3基因。发现三个分离物，包括山梨糖醇发酵大肠杆菌O157：H7，对Colistin具有最小抑制浓度值为128Ω·μg/ ml的表型抗性。山梨糖醇发酵大肠杆菌O157的基因组：H7没有显示出在通用基因组数据库中发现的任何其他基因组序列的100％相似性。还确定该分离物携带62种不同的抗微生物抗性基因。这是质粒介导的MCR-2和MCR-3基因的第一个报告，其携带大肠杆菌O157：H7的牛和绵羊分离物和耐菌抗山梨糖醇发酵大肠杆菌O157：H7的WGS。该研究的结果表明，牛和绵羊可以是大肠杆菌O157：H7中的乳蛋白抗性的重要来源，屠宰场废水可能是传播质粒介导的凉爽基因的重要途径。因此，应仅限于兽医在兽医中使用钙霉素以降低抵抗的发展。此外，可能需要检查流行病学研究中未缺少山梨糖醇发酵大肠杆菌O157：H7的非山梨糖醇发酵的分离方案。

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