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首页> 外文期刊>Microbial Pathogenesis >Antimicrobial activity of Lactobacillus fermentum TcUESCO1 against Streptococcus mutans UA159
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Antimicrobial activity of Lactobacillus fermentum TcUESCO1 against Streptococcus mutans UA159

机译：乳酸杆菌的抗菌活性TCUESCO1对抗链球菌UA159

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Dental caries is a multifactorial chronic-infection disease, which starts with a bacterial biofilm formation caused mainly by Streptococcus mutans. The use of probiotics has shown numerous health benefits, including in the fight against oral diseases. Strains of Lactobacillus fermentum have already shown probiotic potential against S. mutans, but there are still few studies. Thus, the aim of our study was to evaluate the antimicrobial activity of the inoculum and metabolites produced by L. fermentum TcUESC01 against S. mutans UA159. For this, a growth curve of L. fermentum was performed and both the inoculum and the metabolites formed in the fermentation were tested against the growth of S. mutans UA159 in agar diffusion tests, and only its metabolites were tested to determine the minimum inhibitory concentration, minimal bactericidal concentration and inhibition of cell adhesion. Inhibition of biofilm formation, pH drop and proton permeability were also tested with the metabolites. The zone of inhibition began to be formed at 14 h and continued until 16 h. The inoculum containing L. fermentum also showed zone of inhibition. The MIC for the metabolites was 1280 mg/mL and the MBC was obtained with a concentration higher than the MIC equal to 5120 mg/mL. Half of the MIC concentration (640 mg/mL) was required to inhibit S. mutans adhesion to the surface of the microplates. In the biofilm analyzes, the treatment with the metabolites in the tested concentration was not able to reduce biomass, insoluble glucans and alkali soluble compared to the control biofilm (p > 0.05). The metabolites also did not affect acid production and acid tolerance of S. mutans cells in biofilms compared to saline group (p > 0.05). Lactic acid (50.38%) was the most abundant organic acid produced by L. fermentum. This is the first report showing that the metabolites produced by the Lactobacillus fermentum TcUESCO1 have a potential to be used as an antimicrobial agent against S. mutans, showing anti-adherence and bactericidal activity against planktonic cells of S. mutans. Thus, further studies should be carried out in order to better understand the antimicrobial activity of metabolites of L. fermentum TCUESC01.
机译：龋齿是一种多因素慢性感染疾病，其始于主要由链球菌强化引起的细菌生物膜形成。益生菌的使用表明了许多健康益处，包括对抗口腔疾病。乳酸杆菌的菌株已经表现出益生菌对抗S. mutans的潜力，但研究仍有很少的研究。因此，我们的研究目的是评估由L.Fermenturec01对S. mutans UA159产生的Inoculum和代谢物的抗微生物活性。为此，进行了L.Fermentum的生长曲线，并且在琼脂扩散试验中，对发酵中形成的接种物和在发酵中形成的代谢物进行测试，并且测试其代谢物以确定最小抑制浓度，最小的杀菌浓度和细胞粘附的抑制。还用代谢物测试抑制生物膜形成，pH降和质子渗透性。抑制区开始于14小时形成并继续至16小时。含有L.Fermentum的接种物也显示出抑制区。代谢物的MIC为1280mg / ml，得到MBC，浓度高于MIC等于5120mg / ml。需要含有MIC浓度（640mg / ml）的一半来抑制S.Ulans对微孔板表面的粘附。在生物膜分析中，与对照生物膜相比，用测试浓度中的代谢物的处理不能够减少生物量，不溶性葡聚糖和碱溶于溶于溶解物（P> 0.05）。与盐水组相比，代谢物也没有影响生物膜中的S.ulans细胞的酸生产和耐酸性。（p> 0.05）。乳酸（50.38％）是L. Fermentum产生的最丰富的有机酸。这是第一个报道，表明乳酸杆菌发酵乳杆菌产生的代谢物1具有潜在的用作抗微生物剂对抗S. mutans的抗微生物剂，显示出对S. mutans的浮鳞细胞的抗粘附和杀菌活性。因此，应进行进一步的研究，以便更好地理解L.FumestueS01代谢物的抗微生物活性。

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