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首页> 外文期刊>Microbial Pathogenesis >Localization of Pasteurella multocida antigens in the brains of pigs naturally infected with Pasteurellosis revealing a newer aspect of pathogenesis
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Localization of Pasteurella multocida antigens in the brains of pigs naturally infected with Pasteurellosis revealing a newer aspect of pathogenesis

机译：Pasteurella multiocada抗原的定位在自然感染的猪大脑中揭示了发病机制的新方面

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摘要

Pasteurella multocida is an economically important respiratory pathogen of pigs confronting swine industry worldwide. Despite extensive research over the decades, its pathogenesis is still poorly understood. Recent reports have demonstrated the nervous system affection as a newer aspect of pathogenesis by Pasteurella multocida type B:2 in Haemorrhagic Septicemia, but there are no reports of the involvement of nervous system by P. multocida in pigs. Therefore, the study was aimed to explore the neurovirulence of Pasteurella multocida in naturally infected pigs. A total of 15 brains were collected from the natural cases of pig mortality suggestive of Pasteurellosis. Grossly, the leptomeninges were markedly congested and brains were oedematously swollen. Histologically, there was moderate to severe fibrinohaemorrhagic and mononuclear cells exudates present in the leptomeningeal tissue and cerebrospinal spaces. Similar vascular inflammatory lesions (perivascular and per-neuronal) along with gliosis, neuronal degeneration and necrosis were noted in various subanatomical sites of the brain (cerebrum, cerebellum, brainstem and spinal cord). The culture and biochemical tests showed the presence of P. multocida within the brain tissue. P. multocida type specific antibody staining in the brain tissues revealed intense distribution of antigens in the inflammatory exudates of meningeal vessels, neurons, glial cells and endothelial cells of the blood vessels contributing its association with neuropathological lesions. Pasteurella multocida specific PCR amplification of capsular polysaccharide gene yielded 460 bp and multiplex PCR showed the involvement of capsular serogroups A &D. All the isolates showed the presence of 10 genes for virulence factors. The disease confirmation of both serotypes was proven by Koch's postulates using Swiss albino mice. Further, histopathological brain lesions along with the immunohistochemical detection of bacterial antigens were corroborated with natural cases of P. multocida as described above. To the best of our knowledge, we first time report the neuroinvasion of P. multocida in naturally infected pigs.
机译：Pasteulla Multocida是全球猪业面临的猪的经济上重要的呼吸病病理学。尽管在几十年来研究了广泛的研究，但其发病机制仍然很糟糕。最近的报道已经证明，Pasteurella Multiocada型B：2在出血性败血症中的发病机制是发病机制的新方面，但没有报道神经系统在猪中的多型。因此，该研究旨在探讨在天然感染的猪中Pasteurella Multocida的神经血管尿。从猪死亡率的自然案例中收集了共有15个脑袋。粗糙，裂解物显着拥塞，大脑被肿胀。组织学上，存在中度至严重的纤维状发泡症和单核细胞渗出物中存在于髓组织组织和脑脊柱椎间空间中。在脑（大脑，小脑，脑干和脊髓）的各种亚偶偶病位点中，注意到了类似的血管炎症病变（血管炎症和每种神经元）以及神经症，神经元变性和坏死。培养和生化试验显示脑组织内的P. Multiocida。 P.脑组织中的多型型特异性抗体染色揭示了脑膜血管，神经元，神经胶质细胞和血管内皮细胞的炎症渗出物中的抗原分布，导致其与神经病理病变相关。荚膜多糖基因的Pasteurella多型PCR扩增产生460bp和多重PCR显示荚膜血清组A＆D的累积。所有分离株表明存在10个基因的毒力因子。通过瑞士白化小鼠通过Koch的假设证明了两种血清型的疾病。此外，如上所述，用P. Multocida的自然案例证实了组织病理学脑病变以及细菌抗原的免疫组化检测。据我们所知，我们首次报告在天然感染的猪中P. Multocida的神经蛋白。

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