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首页> 外文期刊>Microbial Pathogenesis >The tst gene associated Staphylococcus aureus pathogenicity island facilitates its pathogenesis by promoting the secretion of inflammatory cytokines and inducing immune suppression
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The tst gene associated Staphylococcus aureus pathogenicity island facilitates its pathogenesis by promoting the secretion of inflammatory cytokines and inducing immune suppression

机译：TST基因相关的葡萄球菌致病性岛通过促进炎性细胞因子的分泌和诱导免疫抑制而促进其发病机制

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摘要

Staphylococcus aureus (S. aureus) is an important pathogen causing various limited or systemic infections. Methicillin resistant S. aureus (MRSA) in particular presents a major clinical and public health problem. Toxic shock syndrome toxin-1 (TSST-1) encoded by the gene tst is an important virulence factor of tst positive S. aureus, leading to multi-organ malfunction. However, the mechanism of TSST-1 in pathogenesis is only partly clear. In this study, we investigated the prevalence of the tst gene in clinical isolates of S. aureus. Then, animal experiments were performed to further evaluate the influence of the presence of the tst gene associated Staphylococcus aureus Pathogenicity Island (SaPI) on body weight, serum cytokine concentrations and the bacterial load in different organs. In addition, macrophages were used to analyze the secretion of cytokines in vitro and bacterial survival in the cytoplasm. Finally, pathological analysis was carried out to evaluate organ tissue impairment. The results demonstrated that the prevalence of tst gene was approximately 17.8% of the bacterial strains examined. BALB/c mice infected with tst gene associated SaPI positive isolates exhibited a severe loss of body weight and a high bacterial load in the liver, heart, kidney and spleen. Pathological analysis demonstrated that tissue impairment was more severe after infection with tst gene associated SaPI positive isolates. Moreover, the secretion of IL-6, IL-2 and IL17A by macrophages infected with tst gene associated SaPI positive isolates clearly increased. Notably, IL-6 secretion in BALB/c mice infected with tst gene associated SaPI positive isolates was higher than that in BALB/c mice infected with negative ones. Together, these results indicated that the tst gene associated SaPI may play a critical role in the pathological process of infection via a direct and persistent toxic function, and by promoting the secretion of inflammatory cytokines that indirectly induce immune suppression.
机译：金黄色葡萄球菌（金黄色葡萄球菌）是导致各种有限或全身感染的重要病原体。甲氧西林抗金黄色葡萄球菌（MRSA）尤其存在主要的临床和公共卫生问题。由基因TST编码的毒性休克综合征毒素-1（TSST-1）是TST阳性黄金黄色葡萄球菌的重要毒力因子，导致多器官发生故障。然而，发病机制中TSST-1的机制仅是部分透明的。在这项研究中，我们研究了金黄色葡萄球菌临床分离株中TST基因的患病率。然后，进行动物实验以进一步评估TST基因相关的葡萄球菌的存在对体重，血清细胞因子浓度和不同器官中的细菌载体的存在的影响。此外，巨噬细胞用于分析细胞质中细胞因子的分泌和细胞质在细胞质中的细菌存活。最后，进行了病理分析以评估器官组织损伤。结果表明，TST基因的患病率约为27.8％的细菌菌株。用TST基因感染的Balb / c小鼠相关的SAPI阳性分离物在肝脏，心脏，肾脏和脾脏中表现出严重的体重和高细菌载荷。病理分析表明，用TST基因相关的SAPI阳性分离株感染后，组织损伤更严重。此外，通过感染TST基因相关的SAPI阳性分离株感染IL-6，IL-2和IL17a的分泌明显增加。值得注意的是，用TST基因相关的SAPI阳性分离株感染的Balb / C小鼠中的IL-6分泌率高于受阴性物质感染的BALB / C小鼠中的小鼠。这些结果表明，TST基因相关的SAPI可以通过直接和持续的毒性功能在感染病理过程中发挥关键作用，并通过促进间接诱导免疫抑制的炎性细胞因子的分泌。

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