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首页> 外文期刊>Microbial Pathogenesis >Comparative analysis of the immune response of the wax moth Galleria mellonella after infection with the fungi Cordyceps militaris and Metarhizium robertsii
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Comparative analysis of the immune response of the wax moth Galleria mellonella after infection with the fungi Cordyceps militaris and Metarhizium robertsii

机译：蜡蛾杂志Mellonella感染后免疫应答的比较分析，用真菌冬虫夏草和Metarhizium Robertsii感染

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摘要

Entomopathogenic fungi form different strategies of interaction with their insect hosts. The influence of fungal infection on insect physiology has mainly been studied for generalists (Metarhizium, Beauveria), but studies of specialized teleomorphic species, such as Cordyceps militaris, are rare. We conducted a comparative analysis of the immune reactions of the wax moth Galleria mellonella after injection with blastospores of C. militaris (Cm) and Metarhizium robertsii (Mr) in two doses (400 and 4000 per larva). Cm-injected insects died more slowly and were more predisposed to bacterial infections than Mr-injected insects. It was shown that Cm infection led to a predominance of necrotic death of hemocytes, whereas Mr infection led to apoptotic death of cells. Cm-infected insects produced more dopamine and reactive oxygen species compared to Mr-infected insects. Moreover, Cm injection led to weak inhibition of phenoloxidase activity and slight enhancement of detoxification enzymes compared to Mr-injected insects. Blastospores of Cm that were cultivated in artificial medium (in vitro) and proliferated in wax moth hemolymph (in vivo) were characterized by equal intensity of fluorescence after staining with Calcofluor White. In contrast, Mr blastospores that proliferated in the wax moth had decreased fluorescence intensity compared to Mr blastospores grown in medium. The results showed that insects combat Cm infection more actively than Mr infection. We suggest that Cm uses fewer universal tools of killing than Mr, and these tools are available because of specific interactions of Cm with hosts and adaptation to certain host developmental stages.
机译：昆虫疗法真菌与他们的昆虫宿主形成不同的互动策略。真菌感染对昆虫生理的影响主要是针对一般主义者（Metarhizium，Beauveria）研究的，但研究了专门的大学物种，如冬虫夏草民兵，很少见。我们对用C. Militaris（CM）和Metarhizium robertsii（MR）的囊孢子注射后的蜡蛾Galleria mellonella的免疫反应进行了对比较分析，两剂（每只幼虫400和4000）。 CM注入的昆虫更慢地死亡，并且比注射昆虫更慢地升高到细菌感染。结果表明，CM感染导致血细胞的坏死性死亡，而MR感染导致细胞凋亡死亡。与Mr感染的昆虫相比，CM感染的昆虫产生更多的多巴胺和活性氧。此外，与MR注入的昆虫相比，CM注射导致酚氧化酶活性的较弱抑制，脱氧酶的轻微增强。通过在染色荧光染色后染色后，在人造培养基（体外）中培养的CM胚胎肿瘤（体外）和在蜡蛾血淋巴（体内）的增殖。相比之下，与培养基中生长的胚胎孢子相比，在蜡蛾中增殖的胚胎孢子率下降。结果表明，昆虫比感染先生更积极地对抗CM感染。我们建议CM使用较少的杀戮工具而不是MR，并且由于CM与主机的特定交互和对某些主机发展阶段的适应性，因此可以使用这些工具。

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