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首页> 外文期刊>Microbial Pathogenesis >Experimental pathology of two highly pathogenic H5N1 viruses isolated from crows in BALB/c mice
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Experimental pathology of two highly pathogenic H5N1 viruses isolated from crows in BALB/c mice

机译：两种高度致病H5N1病毒的实验性病理学在Balb / C小鼠中分离的乌鸦

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In this study, we assessed the pathogenicity of two H5N1 viruses isolated from crows in mice. Eighteen 6-8 weeks BALB/c mice each were intranasally inoculated with 10(6) EID50/ml of H5N1 viruses A/crow/India/03CA04/2015 (H9N2-PB2 reassortant H5N1) and A/crow/India/02CA01/2012 (Non-reassortant H5N1). The infected mice showed dullness, weight loss and ruffled fur coat. Histopathological examination of lungs showed severe congestion, haemorrhage, thrombus, fibrinous exudate in perivascular area, interstitial septal thickening, bronchiolitis and alveolitis leading to severe pneumonic changes and these lesions were less pronounced in reassortant virus infected mice. Viral replication was demonstrated in nasal mucosa, lungs, trachea and brain in both the groups. Brain, lung, nasal mucosa and trachea showed significantly higher viral RNA copies and presence of antigen in immunohistochemistry in both the groups. This study concludes that both the crow viruses caused morbidity and mortality in mice and the viruses were phenotypically highly virulent in mice. The H5N1 viruses isolated from synanthropes pose a serious public health concern and should be monitored continuously for their human spill-over.
机译：在这项研究中，我们评估了从小鼠中乌鸦分离的两种H5N1病毒的致病性。 18张6-8周的Balb / c小鼠每种鼻内接种10（6）个EID50 / ml H5N1病毒A / Crow / India / 03CA04 / 2015（H9N2-PB2 Rearsortant H5N1）和A / Crow / India / 02ca01 / 2012 （非重新排列H5N1）。感染的小鼠显示暗沉，减肥和荷叶边的皮肤。肺的组织病理学检查显示严重充血，出血，血栓，纤维血管面积，间隙隔膜增稠，支气管炎和肺炎，导致严重的肺炎变化，在重新排列的病毒感染的小鼠中较不明显。在鼻腔粘膜，肺部，气管和脑中的血清中展示了病毒复制。脑，肺，鼻粘膜和气管在两组中显示出显着更高的病毒RNA拷贝和免疫组织化学中的抗原存在。这项研究得出结论，乌鸦病毒两者都引起小鼠的发病率和死亡率，病毒在小鼠中表现出高度毒性。从Synanthopes中分离的H5N1病毒构成了严重的公共卫生问题，应持续监测他们的人泄漏。

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