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Antitumor effect of exopolysaccharide produced by Bacillus mycoides

机译：芽孢杆菌产生的外糖糖的抗肿瘤作用

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Recently, microbial exopolysaccharides (EPSs) have offered very large field for medical applications owing to their bioactive characteristics. This study aimed to obtain antitumor EPS and to optimize its production using different optimization approaches. Eighty EPSs-producing bacteria were obtained from mud samples. Isolate BS4 was selected as the most potent antitumor EPS-producer and identified as Bacillus mycoides BS4 using 16S rRNA gene sequence analysis. Cell viability and antitumor activity of produced EPS were investigated using microscopic examination and MTT assay. Interestingly, the produced EPS exhibited low cytotoxicity against normal cell baby hamster kidney (BHK) with IC(50 )at 254 mu gml(-1) while it exhibited an inhibitory effect against cancer cells of human hepatocellular carcinoma (HepG2) and Colorectal adenocarcinoma cells (Cato-2) with IC50 of 138 mu gml(-1) and 159 mu gml(-1), respectively. The purified EPS was characterized using Fourier transform infrared, gel permeation chromatography and gas chromatography-mass spectroscopy. It showed molecular weight of 1.90 x 10(4) Da and consists of galactose, mannose, glucose and glucuronic acid. The factors affecting EPS production were optimized using one-factor-at-a time and statistical optimization methods. The Placket-Burman (PB) design results indicated that sugarcane molasses, peptone and shaking conditions were the most significant variables, which were further optimized by Response Surface Methodology (RSM). The optimum conditions for EPS production were 8.0% (w/v) sugarcane molasses, 6 peptone and 300 rpm that produce 8.02gL(-1) of EPS. This indicates the potentiality of Bacillus mycoides BS4 for production of EPS with biomedical applications.
机译：最近，由于其生物活性特性，微生物脱氧糖（EPS）为医疗应用提供了非常大的领域。本研究旨在获得抗肿瘤EPS，并使用不同优化方法优化其生产。从泥浆样品获得八十次epss细菌。将分离物BS4选择为最有效的抗肿瘤EPS-生产者，并使用16S rRNA基因序列分析鉴定为杆菌菌霉菌BS4。使用显微镜检查和MTT测定研究产生的EPS的细胞活力和抗肿瘤活性。有趣的是，所产生的EPS在254μg（-1）时对常规细胞婴儿仓鼠肾（BHK）的低细胞毒性表现出低细胞毒性，而IC（50）在254μg（-1）上，在其肝细胞癌（HepG2）和结肠直肠腺癌细胞上表现出抑制作用（CATO-2）分别具有138μgMML（-1）和159μGML（-1）的IC50。纯化的EPS用傅里叶变换红外，凝胶渗透色谱和气相色谱 - 质谱。它显示分子量为1.90×10（4）DA，由半乳糖，甘露糖，葡萄糖和葡糖醛酸组成。影响EPS生产的因素使用一次性和统计优化方法进行优化。 Placket-Burman（PB）设计结果表明，甘蔗糖蜜，蛋白胨和摇动条件是最重要的变量，其通过响应表面方法（RSM）进一步优化。 EPS生产的最佳条件为8.0％（w / v）甘蔗糖蜜，6个蛋白胨和300rpm，产生8.02g1（-1）的EPS。这表明Bacillus mycoides BS4用于生产具有生物医学应用的EPS的潜力。

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