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首页> 外文期刊>Microbial Pathogenesis >Purinergic signaling and gene expression of purinoceptors in the head kidney of the silver catfish Rhamdia quelen experimentally infected by Flavobacterium columnare
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Purinergic signaling and gene expression of purinoceptors in the head kidney of the silver catfish Rhamdia quelen experimentally infected by Flavobacterium columnare

机译：用香料柱实验感染的银鲶鱼鼠脊柱头肾上腺肾上腺素的嘌呤能信号和基因表达

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摘要

The head kidney is a lymphoid immune organ that plays a key role in the immune and inflammatory responses of teleost fish. It is associated with immunoglobulin G production and differentiation of B cells. The presence of a multi-enzymatic complex found anchored in the plasma membrane makes the head kidney an important purinergic-dependent tissue. Purinergic signaling has been associated with these responses under pathological conditions via regulation of extracellular adenosine triphosphate (ATP), the main damage molecular associated pattern agent released during bacterial infections. The aim of this study was to determine whether purinergic signaling is a pathway associated with impairment of immune responses in silver catfish (Rhamdia quelen) experimentally infected by Flavobacterium columnare, as well as to evaluate the role of P2 purine receptors in this response. Triphosphate diphosphohydrolase (NTPDase) activity in the head kidney was significantly lower in silver catfish experimentally-infected F. columnare 72 h post-infection (hpi) than in the control group, while no significant difference was observed with respect NTPDase activity on adenosine diphosphate, as well as on 5'nucleotidase and adenosine deaminase activities. Extracellular ATP levels were significantly higher in the head kidney of experimentally-infected fish than in the control group at 72 hpi. Finally, p2ty11 and p2rx3 purine receptor levels were significantly higher in experimentally-infected fish than in the control group at 72 hpi. We conclude that purinergic signaling in the head kidney of silver catfish infected by F. columnare creates a proinflammatory profile that may contribute to impairment of immune and inflammatory responses via reduction of ATP hydrolysis and its accumulation in the extracellular milieu, accompanied by upregulation of p2ty11 and p2rx3 purine receptors, leading to pro-inflammatory status.
机译：头肾是一种淋巴的免疫器官，在Textost鱼的免疫和炎症反应中起着关键作用。它与免疫球蛋白G产生和B细胞的分化有关。发现在质膜​​中固定的多酶复合物的存在使头肾成为重要的嘌呤能依赖性组织。在细胞外腺苷三磷酸（ATP）的调节下，嘌呤能信号传导与病理条件下的这些反应有关，在细菌感染期间释放的主要损伤分子相关图案试剂。本研究的目的是确定嘌呤能信号传导是否是与通过香脂钾柱的银鲶（rhamdia Quelen）中的免疫应答损伤有关的途径，以及评估P2嘌呤受体在这种反应中的作用。在肾脏的三磷酸二磷酸二磷酸酶（NTPDase）活性在银鲶实验上感染的F. PlaseArite 72 H后显着低于对照组（HPI），而在腺苷二磷酸腺苷的NTPDase活性没有观察到显着差异，以及5'核苷酸酶和腺苷脱氨酶活性。在72hPi的实验感染鱼头肾脏的细胞外ATP水平显着高于对照组。最后，在实验感染的鱼中，P2TY11和P2RX3嘌呤受体水平显着高于72hPI的对照组。我们得出结论，由F. Collumary的银鲶头肾脏中的纯化信号传导造成促炎剖面，可以通过减少ATP水解及其在细胞外Milieu中的积累有助于免疫和炎症反应的损害，并伴随着P2Ty11的上调和P2RX3嘌呤受体，导致促炎状态。

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