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首页> 外文期刊>Microbial Pathogenesis >Molecular characterization of virulence and drug resistance genes-producing Escherichia coli isolated from chicken meat: Metal oxide nanoparticles as novel antibacterial agents
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Molecular characterization of virulence and drug resistance genes-producing Escherichia coli isolated from chicken meat: Metal oxide nanoparticles as novel antibacterial agents

机译：从鸡肉中分离的毒力和药物抗性基因的分子表征 - 金属氧化物纳米粒子作为新型抗菌剂

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Escherichia coli is a major global foodborne pathogen, infecting a wide range of animals and contaminating their meat products. E. coll., can lead to high morbidity and mortality with a huge economic loss especially if food-borne diseases are associated with multidrug resistant (MDR)- and multivirulent-producing pathogens. Due to the increased resistance to common antimicrobials used to treat livestock animals and human infections, the discovery of new and innovative nanomaterials are in high demand. Recently, metal oxides can be considered as effective inorganic agents with antimicrobial features. Hence, this study might be the first to evaluate the ef-ficiency of metal oxide nanoparticles (MO-NPs) as novel antibacterial agents against MDR/multivirulent E. coli pathogens isolated from chicken meat. The occurrence of pathogenic E. coli was determined in fresh warm chicken meat parts (breast, thigh, liver and gizzard). Ninety-one of 132 (69%) chicken meat parts were Escherichia -positive with E. coli as the only species isolated. Out of identified 240 E. coll. strains, 72.5% (174/240) were classified as MDR E. coll. strains. Fifty-five profile patterns were obtained. From each pattern, one strain was randomly selected for further analysis of virulence and resistance genes. Extracted DNA was assessed for the presence of antibiotic resistance genes (bla(IMP-7 ),bla(IMP-25), bla(TEM), bla(SHV), bla(OXA-2), tetA, aadA, and aac(3)-IV) and virulence genes (stx1, stx2, hlyA, eaeA, aggR, eltB, estib, papA, afa and hlyD). Clustering analyses revealed that 10 E. coli harboring the highest number of virulence and resistance genes were shifted together into one cluster designated as cluster X. The average activities of zinc peroxide nanoparticles (ZnO2-NPs) were higher than that of zinc oxide nanoparticles (ZnO-NPs) and titanium dioxide nanoparticles (TiO2-NPs) by 20% and 29%, respectively. The anti-inflammatory activity of ZnO2-NPs in comparison with aspirin was assessed using mem-brane stabilization, albumin denaturation, and proteinase inhibition methods. Significant anti-inflammatory activity of ZnO2-NPs was achieved at concentration levels of 500-1000 mu g/ml. It seems that MO-NPs are effective alternative agents, since they exhibited a competitive antibacterial capability against MDR/multivirulent-pro-ducing E. coli pathogens isolated from chicken meat. Hence, ZnO2-NPs are a promising nanoparticles-based material for controlling foodborne pathogens, thereby valued for food safety applications.
机译：大肠杆菌是一种主要的全球性食源性病原体，感染了各种各样的动物并污染了他们的肉类产品。 E. Coll。，可能导致高度发病率和死亡率，特别是如果食物传播疾病与多药抗性（MDR）相关 - 以及产生多种多变的病原体。由于对用于治疗牲畜动物和人类感染的常见抗微生物的抗性增加，对新的和创新的纳米材料的发现是高需求。最近，金属氧化物可以被认为是具有抗微生物特征的有效无机剂。因此，该研究可能是第一种评价金属氧化物纳米颗粒（MO-NPS）作为针对与鸡肉中分离的MDR / Multivorione大肠杆菌病原体的新型抗菌剂的EF型抗菌剂。在新鲜温暖的鸡肉零件（乳房，大腿，肝脏和Gizzard）中测定致病大肠杆菌的发生。 132名中的九十六（69％）鸡肉肉部分是大肠杆菌 - 与孤立的唯一物种为大肠杆菌。识别240 E. Coll。菌株72.5％（174/240）被分类为MDR E. Coll。菌株。获得了五十五种概况模式。从每种图案中，随机选择一种菌株以进一步分析毒力和抗性基因。评估提取的DNA用于抗生素抗性基因（BLA（IMP-7），BLA（IMP-25），BLA（TEM），BLA（SHV），BLA（OXA-2），TETA，AAD和AAC（ 3）-IV）和毒力基因（STX1，STX2，Hlya，EAEA，Z，ELTB，estib，爸爸，AFA和Hlyd）。聚类分析显示，含有最多的毒力和抗性基因的10e.coli被转移到指定为簇X的一个簇中。过氧化锌纳米颗粒（ZnO2-NPS）的平均活性高于氧化锌纳米颗粒（ZnO） -NPS）和二氧化钛纳米颗粒（TiO 2 -NP）分别为20％和29％。使用Mem-Brans稳定化，白蛋白变性和蛋白酶抑制方法评估与阿司匹林相比的ZnO2-NP的抗炎活性。 ZnO2-NPS的显着抗炎活性在500-1000μmg/ ml的浓度水平下实现。似乎Mo-NPS是有效的替代药物，因为它们表现出针对与鸡肉中分离的MDR / Multivihulion-Pro-Diologens的竞争性抗菌能力。因此，ZnO2-NPS是一种有前途的基于纳米颗粒的材料，用于控制食源性病原体，从而赋予食品安全应用。

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