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首页> 外文期刊>Microbial Pathogenesis >Analysis of different signal peptides for the secretory production of Ama r 2 in gram-positive systems (Lactococcus lactis)
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Analysis of different signal peptides for the secretory production of Ama r 2 in gram-positive systems (Lactococcus lactis)

机译：革兰阳性系统中AMA R 2分泌生产不同信号肽的分析（乳球菌乳酸）

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摘要

Prokaryotic systems have been considered the most affordable and simplest hosts which are being employed to express recombinant proteins such as allergens; nevertheless, without appropriate signal peptide (SP), these systems cannot be used for secretory proteins. Recently, a lot of effort has been put into assessing the potential of gram-positive strains such as lactic acid bacteria for new applications in the production of heterologous proteins. Ama r 2 is a respiratory allergen from Amaranthus retroflexus, whose recombinant production in the probiotic host could be introduced as a specific and effective way to rapid diagnosis and immunotherapy of this allergy. Consequently, the production of this recombinant protein using the prokaryotic system, requires a suitable SP to protect disulfide bonds and to prevent misfolding. This study was designed to predict the best SPs for the expression of Ama r 2 protein in Lactococcus lactis as the host. In this study, 42 signal sequences were selected from SP databases and the most important features of them were evaluated. First, n, h and c regions of the SPs and their probabilities were investigated by signalP software version 4.1. Then, their physicochemical properties were evaluated by Portparam and SOLpro. Moreover, the secretion sorting and sub-cellular localization sites were evaluated by PRED-TAT and ProtcompB software programs. The results revealed that yjgB, entC2 (Entrotoxine type C-2), ent B (Entrotoxine type), blaZ (Beta lactamase), dex (number 21), blm (Beta lactamase 2), dex (Dextranase; number 20) and number 26 were introduced theatrically as the best SPs to express Ama r 2 in Lactococcus lactis.
机译：原核系统被认为是用于表达过敏原等重组蛋白的最实惠和最简单的宿主;然而，没有适当的信号肽（SP），这些系统不能用于分泌蛋白。最近，很多努力已经评估了革兰氏阳性菌株的潜力，例如乳酸菌，用于在异源蛋白的生产中的新应用。 AMA R 2是来自Amaranthus Retroflexus的呼吸变性，其益生菌宿主中的重组产量可以作为这种过敏的快速诊断和免疫治疗的特定有效的方法。因此，使用原核系统的这种重组蛋白的产生需要合适的SP来保护二硫键并防止误折叠。本研究旨在预测乳酸乳乳乳乳杆菌作为宿主中AMA R 2蛋白表达的最佳SP。在该研究中，从SP数据库中选择了42个信号序列，并评估它们的最重要的特征。首先，SIGNEP软件版本4.1研究了SPS的N，H和C区域及其概率。然后，它们的物理化学性质由PortParam和Solpro评估。此外，通过PRED-TAT和PRODCOMPB软件程序评估分泌分类和子蜂窝定位站点。结果表明，YJGB，ENTC2（Entrotoxine C-2），ENT B（entrotoxine型），Blaz（β内酰胺酶），DEX（21），BLM（β内酰胺酶2），DEX（葡聚糖酶;数20）和数量26术语作为最佳SPS在乳酸乳乳杆菌中表达AMA R 2的最佳SP。

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