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首页> 外文期刊>Microbial Pathogenesis >Microbiological profile of distinct virulence of Nocardia cyriacigeorgica strains in vivo and in vitro
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Microbiological profile of distinct virulence of Nocardia cyriacigeorgica strains in vivo and in vitro

机译：体内和体外诺卡迪亚金刚虫岛菌株的微生物概况

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摘要

There are significant differences between different Nocardia species regarding geographical distribution, biochemical features, phenotypic characterization, and drug sensitivity. In this study, we explored the differences in virulence and pathogenic mechanisms of two Nocardia cyriacigeorgica strains. We examined the difference in virulence between N. cyriacigeorgica ATCC14759 and N. cyriacigeorgica GUH-2 by measuring cytotoxicity, animal survival after infection, the ability of host cell invasion, and viability in host cells. Western blotting was used to compare the differences in activation of MAPKs, including p38, ERK, and JNK, the NF-kappa B signaling pathway, and the PI3K/Akt signaling pathway in A549 and RAW264.7 cells. We measured the difference in stimulatory effects on production of the cytokines IL-6, IL-10, and TNF-alpha by ELISA. We found that N. cyriacigeorgica ATCC14759 causes higher cytotoxicity in cultured cells and higher lethality in mice, and exhibits superior invasion ability and viability in host cells compared with N. cyriacigeorgica GUH-2. Moreover, these two strains show marked differences in activation of the expression of cytokines and signaling pathways. N. cyriacigeorgica ATCC14759 is more virulent than N. cyriacigeorgica GUH-2. Furthermore, there is a significant difference in pathogenesis between the two strains. Our results provide a theoretical basis for the prevention and treatment of Nocardia infection.
机译：关于地理分布，生化特征，表型表征和药物敏感性的不同Nocardia物种之间存在显着差异。在这项研究中，我们探讨了两种Nocardia Cyriadigeorga菌株的毒力和致病机制的差异。通过测量细胞毒性，感染后动物存活，宿主细胞侵袭能力，在宿主细胞侵袭的能力，检测N.CyriaciGeorgica ATCC14759和N.Cyriacigeorgica Guh-2之间的毒力差异。 Western Blotting用于比较MAPK的激活差异，包括P38，ERK和JNK，NF-KAPPA B信号通路和A549和RAW264.7细胞中的PI3K / AKT信号传导途径。我们测量了ELISA对细胞因子IL-6，IL-10和TNF-α产生的刺激作用的差异。我们发现N.Cyriacigeorgica ATCC14759在培养细胞中引起更高的细胞毒性，并在小鼠中较高，并且与N.CyRiacigeorgica Guh-2相比，宿主细胞中的卓越侵袭能力和活力。此外，这两种菌株显示出明显的差异，激活细胞因子和信号通路的表达。 N.Cyriacigeorgica ATCC14759比N.Cyriacigeorgica Guh-2更具毒性。此外，两种菌株之间的发病机制存在显着差异。我们的结果为预防和治疗Nocardia感染提供了理论依据。

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