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首页> 外文期刊>Microbial Pathogenesis >A trivalent vaccine consisting of 'flagellin A plus B and pilin' protects against Pseudomonas aeruginosa infection in a murine burn model
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A trivalent vaccine consisting of 'flagellin A plus B and pilin' protects against Pseudomonas aeruginosa infection in a murine burn model

机译：由“鞭毛蛋白A加B和Pilin”组成的三价疫苗，防止鼠烧伤模型中的假单胞菌铜绿假单胞菌感染

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Pseudomonas aeruginosa is a common nosocomial pathogen in burn patients, and rapidly achieves antibiotic resistance, and thus, developing an effective vaccine is critically important for combating P. aeruginosa infection. Flagella and pili play important roles in colonization of P. aeruginosa at the burn wound site and its subsequent dissemination to deeper tissue and organs. In the present study, we evaluated protective efficacy of a trivalent vaccine containing flagellins A and B (FlaA + FlaB) + pilin (PilA) in a murine burn model of infection. "FlaA + FlaB + PilA" induced greater protection in P. aeruginosa murine burn model than the single components alone, and it showed broad immune protection against P. aeruginosa strains. Immunization with "FlaA + FlaB + PilA" induced strong opsonophagocytic antibodies and resulted in reduced bacterial loads, systemic IL-12/IL-10 cytokine expression, and increased survival after challenge with three times lethal dose fifty (LD50) of P. eruginosa strains. Moreover, the protective efficacy of "FlaA + FlaB + PilA" vaccination was largely attributed to specific antibodies. Taken together, these data further confirm that the protective effects of "FlaA + FlaB + PilA" vaccine significantly enhance efficacy compared with antibodies against either mono or divalent antigen, and that the former broadens the coverage against P. eruginosa strains that express two of the three antigens.
机译：假单胞菌铜绿假单胞菌是烧伤患者的常见医院病原体，迅速实现抗生素耐药性，因此，显影有效的疫苗对于打击铜绿假单胞菌感染至关重要。鞭毛和皮里在燃烧伤口部位的P.铜绿假单胞菌定植中的重要作用及其随后的播种为更深的组织和器官。在本研究中，我们在鼠烧伤模型中评估了含有鞭毛A和B（FLAA + Blab）+ Pilin（Pila）的鞭毛疫苗的保护效果。 “FLAA + Flab + Pila”在P. Aerginosa鼠燃烧模型中诱导更高的保护，而不是单独的单一组分，并且它显示出对铜绿假单胞菌菌株的宽免疫保护。用“FLAA + Flab + Pila”诱导强大的Opsonococytic抗体免疫，并导致细菌载荷减少，全身IL-12 / IL-10细胞因子表达，并在致命致死的P.Fuginosa菌株的致命剂量五十（LD50）攻击后增加存活率。此外，“FLAA + FLAB + PLA”疫苗接种的保护效率主要归因于特异性抗体。与众不同，这些数据进一步证实，与抗针对单级或二价抗原的抗体相比，“FLAA + Flab + Pila”疫苗的保护作用显着提高了疗效，并且前者拓宽了表达两种三个抗原。

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