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首页> 外文期刊>Micron: The international research and review journal for microscopy >New approach to electron microscopy imaging of gel nanocomposites in situ
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New approach to electron microscopy imaging of gel nanocomposites in situ

机译：凝胶纳米复合材料原位电子显微镜成像的新方法

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Characterization of Au-nanocomposites is routinely done with scattering techniques where the structure and ordering of nanoparticles can be analyzed. Imaging of Poloxamer gel-based Au-nanocomposites is usually limited to cryo-TEM imaging of cryo-microtomed thin sections of the specimen. While this approach is applicable for imaging of the individual nanoparticles and gauging their size distribution, it requires altering the state of the specimen and is prone to artifacts associated with preparation protocols. Use of Scanning Transmission Electron Microscopy (S/TEM) with fluid cell in situ provides an opportunity to analysis of these complex materials in their hydrated state with nanometer resolution, yet dispensing dense gel-based samples onto electron-transparent substrates remains challenging. We show that Poloxamer gel-based Au nanocomposites exhibiting thermo-reversible behavior can be imaged in a fully hydrated state using a commercially available fluid cell holder, and we describe a specimen preparation method for depositing femtoliter amounts of gel-based nanocomposites directly onto the 50 nm-thick SiN window membranes. Ultimately, fluid cell S/TEM in situ imaging approach offers a pathway to visualization of individual nanoparticles within a thick gel media while maintaining the hydrated state of the carrier polymeric matrix.
机译：通过散射技术常规地完成Au-NaNOC复合材料的表征，其中可以分析纳米颗粒的结构和排序。泊洛沙姆凝胶基Au-纳米复合材料的成像通常限于样本的低温微量薄截面的Cryo-TEM成像。虽然这种方法适用于单个纳米颗粒的成像并测量其尺寸分布，但是它需要改变样本的状态，并且容易伴随与制剂方案相关的伪影。用原位使用流体电池的扫描透射电子显微镜（S / TEM）提供了利用纳米分辨率的水合状态分析这些复合材料的机会，但将致密的凝胶基样品分配到电子透明基板上保持具挑战性。我们表明，使用市售的流体电池支架，可以在完全水合状态下对具有热可逆行为的泊洛沙姆的基于凝胶的Au纳米复合材料，并且我们描述了一种用于将Femtoliter大量的凝胶基纳米复合材料沉积到50上的样品制备方法NM厚的SIN窗膜。最终，流体电池S / TEM以原位成像方法提供了一种通向厚凝胶介质内的单独纳米颗粒的途径，同时保持载体聚合物基质的水合状态。

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