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首页> 外文期刊>Mikrochimica Acta: An International Journal for Physical and Chemical Methods of Analysis >An aptamer based fluorometric assay for amyloid-beta oligomers using a metal-organic framework of type Ru@MIL-101(Al) and enzyme-assisted recycling
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An aptamer based fluorometric assay for amyloid-beta oligomers using a metal-organic framework of type Ru@MIL-101(Al) and enzyme-assisted recycling

机译：使用ru @ mil-101（Al）的金属 - 有机框架和酶辅助回收的金属 - 有机骨架的淀粉样蛋白β低聚物的荧光测定法

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摘要

Amyloid-beta (A beta) oligomers causing neuron damage are regarded as potential therapeutic targets and diagnostic markers for Alzheimer's disease (AD). A homogeneous turn-on fluorometric aptasensor is described for A beta oligomers. It is highly selective and non-invasive and based on (a) the use of a luminescent metal-organic framework carrying aptamer-modified AuNPs (L-MOF/Apt-Au) as tracking agent, and (b) enzyme-assisted target recycling signal amplification. The tracking agent does not emit fluoresce by fluorescence resonance energy transfer (FRET) between the luminescent MOF as donor and Apt-Au as the acceptor under the excitation wavelength of 466 nm. When A beta oligomers are added to the tracking agent solution, the Apt-Au on tracking agent can preferentially bind with A beta oligomers and then be released. This turns the "off" signal of the luminescent MOF tracer to the "on" state. The enzyme (Rec Jf exonuclease) added into the supernatant further improves sensitivity due to enzyme-assisted target-recycling signal amplification. The assay has an excellent linear response to A beta oligomers from 1.0 pM to 10 nM, with a detection limit of 0.3 pM. This homogeneous turn-on fluorometric method is expected to have potential and applications in clinical diagnosis.
机译：导致神经元损伤的淀粉样蛋白-β（β）低聚物被认为是阿尔茨海默病（AD）的潜在治疗靶标和诊断标志物。 β低聚物描述了均匀的开启荧光Aptasensor。它是高度选择性和非侵入性的，并且基于（a）使用携带适体改性的aUnps（L-Mof / apt-au）作为跟踪剂的发光金属 - 有机骨架，和（b）酶辅助目标回收信号放大。跟踪剂不会在发光MOF之间作为供体之间的荧光共振能量转移（FRET）发荧光，并且APT-Au作为受激发波长为466nm的受体。当将β低聚物添加到跟踪剂溶液中时，跟踪剂上的APT-Au可以优先与β低聚物结合，然后被释放。这将发光MOF示踪剂的“OFF”信号转变为“ON”状态。添加到上清液中的酶（REC JF外切核酸酶）进一步提高了酶辅助靶 - 再循环信号扩增的敏感性。该测定与1.0pm至10nm的β低聚物具有优异的线性响应，检测限为0.3μm。这种均匀的开启荧光法预计在临床诊断中具有潜力和应用。

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