Three-dimensional structure of phosphoribosyl pyrophosphate synthetase from E-coli at 2.71 angstrom resolution

Timofeev V. I.; Abramchik Yu. A.; Zhukhlistova N. E.; Muravieva T. I.; Esipov R. S.; Kuranova I. P.

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Three-dimensional structure of phosphoribosyl pyrophosphate synthetase from E-coli at 2.71 angstrom resolution

机译：大肠杆菌中2.71埃分辨率的磷酸核糖焦磷酸合成酶的三维结构

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Phosphoribosyl pyrophosphate synthetase from Escherichia coli was cloned, purified, and crystallized. Single crystals of the enzyme were grown under microgravity. The X-ray diffraction data set was collected at the Spring-8 synchrotron facility and used to determine the three-dimensional structure of the enzyme by the molecular-replacement method at 2.71 resolution. The active and regulatory sites in the molecule of E. coli phosphoribosyl pyrophosphate synthetase were revealed by comparison with the homologous protein from Bacillus subtilis, the structure of which was determined in a complex with functional ligands. The conformations of polypeptide-chain fragments surrounding and composing the active and regulatory sites were shown to be identical in both proteins.

机译：克隆，纯化和结晶了来自大肠杆菌的磷酸核糖焦磷酸合成酶。酶的单晶在微重力下生长。 X射线衍射数据集是在Spring-8同步加速器设施中收集的，用于通过分子置换方法以2.71分辨率确定酶的三维结构。通过与枯草芽孢杆菌的同源蛋白进行比较，揭示了大肠杆菌磷酸核糖焦磷酸合成酶分子中的活性和调控位点，该蛋白的结构是在具有功能性配体的复合物中确定的。在两种蛋白质中，围绕并组成活性和调节位点的多肽链片段的构象均显示为相同。

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《Crystallography reports》 |2016年第1期|共11页
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Timofeev V. I.; Abramchik Yu. A.; Zhukhlistova N. E.; Muravieva T. I.; Esipov R. S.; Kuranova I. P.;
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正文语种 eng
中图分类晶体学;
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1. Three-dimensional structure of phosphoribosyl pyrophosphate synthetase from E-coli at 2.71 angstrom resolution [J] . Timofeev V. I., Abramchik Yu. A., Zhukhlistova N. E., Crystallography reports . 2016,第1期

机译：大肠杆菌中2.71埃分辨率的磷酸核糖焦磷酸合成酶的三维结构
2. The structures of Thermoplasma volcanium phosphoribosyl pyrophosphate synthetase bound to ribose-5-phosphate and ATP analogs. [J] . Cherney MM, Cherney LT, Garen CR, Journal of Molecular Biology . 2011,第4期

机译：质热体火山磷酸核糖基焦磷酸合成酶的结构与5磷酸核糖和ATP类似物结合。
3. STRUCTURE AND FUNCTION OF S-ADENOSYLMETHIONINE SYNTHETASE - CRYSTAL STRUCTURES OF S-ADENOSYLMETHIONINE SYNTHETASE WITH ADP, BRADP, AND PPI AT 2.8 ANGSTROM RESOLUTION [J] . Takusagawa F, Kamitori S, Markham GD Biochemistry . 1996,第8期

机译：S-腺苷甲硫氨酸合成酶的结构和功能-在2.8角分辨率下具有ADP，BRADP和PPI的S-腺苷甲硫氨酸合成酶的晶体结构
4. Three-dimensional angstrom resolution in fluorescence microscopy: Insight into protein structure [C] . Daniel Boening, Siegfried Weisenburger, Benjamin Schomburg, Conference on Lasers and Electro-Optics Europe;European Quantum Electronics Conference . 2017

机译：荧光显微镜中的三维埃分辨率：深入了解蛋白质结构
5. Crystal structures of two plasmid copy-control-related RNA duplexes: An 18-base-pair duplex at 1.20 angstrom resolution and a 19-base-pair duplex at 1.55 angstrom resolution [D] . Klosterman, Peter Scott 1999

机译：两个质粒复制控制相关RNA双链体的晶体结构：1.20埃分辨率的18个碱基对的双链体和1.55埃分辨率的19个碱基对的双链体
6. The Refined Three-Dimensional Structure of Pectate Lyase C from Erwinia chrysanthemi at 2.2 Angstrom Resolution (Implications for an Enzymatic Mechanism). [O] . M. D. Yoder, F. Jurnak 1995

机译：菊花欧文氏菌的果胶裂合酶C的精制三维结构分辨率为2.2埃（对酶机制的影响）。
7. Purification of rat liver phosphoribosyl pyrophosphate synthetase and studies on the structure and properties of the enzyme component [O] . 喜多和子, Kita Kazuko, キタカズコ 100

机译：大鼠肝磷酸核糖焦磷酸合成酶的纯化及酶组分的结构和性质研究
8. Photoelectric Atlas of the Intense Lines of the Hydrogen Molecular Emission Spectrum from 1025 to 1650Angstrom to 1650Angstrom at a Resolution of 0.10Angstrom [R] . Schubert, K. E., Hudson, R. D. 1963

机译：氢分子发射光谱强烈线的光电图谱从1025到1650埃到1650埃，分辨率为0.10埃

Three-dimensional structure of phosphoribosyl pyrophosphate synthetase from E-coli at 2.71 angstrom resolution

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