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首页> 外文期刊>Molecular ecology resources >A chromosome-level genome assembly reveals the genetic basis of cold tolerance in a notorious rice insect pest, Chilo suppressalis
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A chromosome-level genome assembly reveals the genetic basis of cold tolerance in a notorious rice insect pest, Chilo suppressalis

机译：染色体级基因组组件揭示了臭虫抑制寒冷耐寒性的遗传基础，Chilo Suppressalis

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摘要

The rice stem borer, Chilo suppressalis, is one of the most damaging insect pests to rice production worldwide. Although C. suppressalis has been the focus of numerous studies examining cold tolerance and diapause, plant-insect interactions, pesticide targets and resistance, and the development of RNAi-mediated pest management, the absence of a high-quality genome has limited deeper insights. To address this limitation, we generated a draft C. suppressalis genome constructed from both Illumina and PacBio sequences. The assembled genome size was 824.35 Mb with a contig N-50 of 307 kb and a scaffold N-50 of 1.75 Mb. Hi-C scaffolding assigned 99.2% of the bases to one of 29 chromosomes. Based on universal single-copy orthologues (BUSCO), the draft genome assembly was estimated to be 97% complete and is predicted to encompass 15,653 protein-coding genes. Cold tolerance is an extreme survival strategy found in animals. However, little is known regarding the genetic basis of the winter ecology of C. suppressalis. Here, we focused our orthologous analysis on those gene families associated with animal cold tolerance. Our finding provided the first genomic evidence revealing specific cold-tolerant strategies in C. suppressalis, including those involved in glucose-originated glycerol biosynthesis, triacylglycerol-originated glycerol biosynthesis, fatty acid synthesis and trehalose transport-intermediate cold tolerance. The high-quality C. suppressalis genome provides a valuable resource for research into a broad range of areas in molecular ecology, and subsequently benefits developing modern pest control strategies.
机译：水稻干螟，辣椒抑制，是全球稻米生产最有害的害虫之一。虽然C.抑制的焦点是众多研究检查耐寒性和滞育，植物 - 昆虫相互作用，农药靶标和抗性，以及RNAi介导的害虫管理的发展，没有高质量的基因组的洞察力有限。为了解决这些限制，我们产生了由Illumina和Pacbio序列构成的C.抑制基因组草案。组装的基因组大小为824.35mb，具有307kb的Contig N-50和1.75mb的支架N-50。 Hi-C脚手架将99.2％的基础分配给29个染色体之一。基于通用单拷贝正端（Busco），估计基因组组件草案均为97％，预计涵盖15,653个蛋白质编码基因。耐寒性是动物中发现的极端生存策略。然而，对于C.抑制的冬季生态学的遗传基础知之甚少。在这里，我们将我们的正交分析专注于与动物耐寒耐受相关的那些基因家族。我们的发现提供了第一种基因组证据，揭示了C.抑制的特异性耐冷耐热策略，包括参与葡萄糖源甘油生物合成的人，三酰基甘油源性甘油生物合成，脂肪酸合成和海藻糖转运中间耐寒耐药性。高质量的C.抑制基因组为研究分子生态学的广泛领域提供了有价值的资源，随后利用发展现代害虫控制策略。

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