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首页> 外文期刊>Mycologia >In vitro virulence determinants, comparative pathogenicity of Diutina (Candida) mesorugosa clinical isolates and literature review of the D. rugosa complex
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In vitro virulence determinants, comparative pathogenicity of Diutina (Candida) mesorugosa clinical isolates and literature review of the D. rugosa complex

机译：体外毒力决定因素，DIUTINA（Candida）中霉菌的比较致病性临床分离物和D.Rugosa综合体的文献综述

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摘要

Opportunistic mycoses by yeasts have increased considerably in the last three decades. Although Candida albicans is considered one of the most important causes of nosocomial infections, there is a recent shift to non-albicans Candida species as the most frequently isolated yeasts in particular risk groups. Diutina rugosa (formerly Candida rugosa) is a complex that includes four species: D. rugosa sensu stricto, D. neorugosa, D. pseudorugosa, and D. mesorugosa, and they are estimated to represent 0.2% of all Candida clinical isolates. In this study, we analyze nine clinical isolates of D. mesorugosa with focus on the virulence determinants and pathogenicity of the species by means of a Galleria mellonella survival model. Overall, we detected very strong aspartyl-protease and esterase activities. In contrast, both DNase and hemolysin activities were evident in only two of the isolates. None of the isolates was positive for phospholipase activity. All isolates studied were able to form biofilm after 72 h of incubation in a robust manner when compared with the C. albicans strain used as control. Susceptibility testing showed minimum inhibitory concentrations (MICs) = 1 mu g/mL for amphotericin B in all isolates tested. Eight out of nine of the isolates had MICs = 2 mu g/mL for fluconazole. All isolates were resistant to both anidulafungin and caspofungin (MICs >= 1 mu g/mL). We found a significant difference (P 0.0001) amongst the survival curves for the different D. mesorugosa isolates in the Galleria mellonella survival model. Strains HPM309 and H259 produced an acute infection and exhibited the highest virulence, whereas the D. mesorugosa isolates 99-480 and DM17 proved to be the less virulent strains.
机译：在过去的三十年中，酵母的机会主义肌肉会增加。虽然念珠菌被认为是医院感染最重要的原因之一，但最近对非蛋白质人的念珠菌物种作为最常见的酵母，近期转变为特别是风险群体。 Diutina Rugosa（原念珠菌）是一个包含四种物种的综合体：D.Rugosa Sensu Sticalo，D. Neorugosa，D.Pseudorugosa和D.Mesorugosa，它们估计占所有念珠菌临床分离物的0.2％。在这项研究中，我们通过Galleria Mellonella存活模型分析了D. Mesorugosa的九个临床分离株D. Mesorugosa的毒力决定因素和致病性。总体而言，我们检测到非常强烈的阿巴罗基蛋白酶和酯酶活性。相反，只有两种分离株都是明显的。磷脂酶活性没有分离物是阳性的。与用作对照的C. albicans菌株相比，在72小时后，所研究的所有分离物能够以稳健的方式孵育72小时后形成生物膜。易感性测试显示最小抑制浓度（MICS）＆在所有分离物中用于两性霉素B的均匀抑制浓度（麦克风）。分离物中的八个含有MICS＆氟康唑的麦克风。所有分离物均对Anidulafungin和Caspofungin（MICS> =1μg/ ml）耐药。我们发现了Galleria Mellonella生存模型的不同D. Mesorugosa分离物的生存曲线中的显着差异（p＆ 0.0001）。菌株HPM309和H259产生了急性感染并表现出最高的毒力，而D. Mesorugosa分离株99-480和DM17被证明是较低的毒性菌株。

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