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首页> 外文期刊>Mycologia >Offspring analysis using two cleaved amplified polymorphic sequence (CAPS) markers reveals amphithallism in the edible mushroom Agaricus sinodeliciosus
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Offspring analysis using two cleaved amplified polymorphic sequence (CAPS) markers reveals amphithallism in the edible mushroom Agaricus sinodeliciosus

机译：使用两种切割的扩增多晶序列（盖子）标记的后代分析揭示了可食用蘑菇姬松茸中的两性术。

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摘要

Agaricus sinodeliciosus is an edible wild mushroom known in northwest China. It belongs to Agaricus section Bivelares that includes several popular cultivated species, such as A. bisporus, the button mushroom. The life cycle of the latter species has been described as amphithallic because both homokaryotic (n) and heterokaryotic (n+n) spores are produced that lead to heterothallic and pseudohomothallic life cycles, respectively. The type of life cycle can impact population structures and breeding strategies. The main objective of this study was to identify the different categories of spores produced by A. sinodeliciosus. Using either a morphological approach based on the number of sterigmata per basidium or a genetic approach based on the genotypes of the progeny at two loci, the proportion of heterokaryotic spores was estimated at 6% and 15%, respectively. Two codominant markers were chosen from the mitochondrial intermediate peptidase gene (MIP) and the nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) region. Genotypic analysis and mating tests confirmed that in A. sinodeliciosus, MIP is centromere-linked and tightly linked to the mating type locus as in A. bisporus and A. subrufescens. We conclude that A. sinodeliciosus has a unifactorial system of sexual incompatibility and an amphithallic life cycle that is predominantly heterothallic, and that its pseudohomothallism follows a nonrandom model with nonsister postmeiotic nuclei paired in the same spore, which give rise to a potentially fertile heterokaryon. This method of using two informative markers is reliable not only in selecting the homokaryotic offspring but also in classifying the homokaryons in two breeding stocks according to their mating type alleles.
机译：Agaricus sinodeliciosus是一个在中国西北部的食用野生蘑菇。它属于姬松茸部分多群，其中包括几种流行的栽培物种，例如Bisporus，按钮蘑菇。后一种物种的生命周期已被描述为氨基allic，因为产生了同源核糖（N）和异核（N + N）孢子，其分别导致杂种属和伪致命的寿命循环。生命周期的类型可以影响人口结构和育种策略。本研究的主要目标是识别由A.Inodeliciosus产生的不同类别的孢子。使用基于每条碱基的菌射数的形态学方法或基于两个基因座的基因分型基因型或基因型方法，估计在6％和15％的特性孢子的比例分别为6％和15％。从线粒体中间肽酶基因（MIP）和NUC RDNA内转录的间隔物（IT1-5.8S-ITS2 = ITS）区域中选择两种Codominant标志物。基因型分析和交配试验证实，在A.Inodeliciosus中，MIP是用Centromere连接的，与配合型位点紧密相关，如在A. bisporus和A. subruffescens中。我们得出结论，A. Sinodeliciosus具有巨大的性不相容性和含有杂种的含有含有杂种的常规生命周期，并且其伪肝功能均遵循与在同一孢子中配对的非经济型后核的非谐波模型，这导致潜在的肥沃的异质核。这种使用两个信息标记的方法不仅可以根据选择同源性后代，而且可以根据它们的交配型等位基因在两种繁殖股中分类同源物中。

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