...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Mycologia >Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition
【24h】

Response of the sesquiterpene synthesis in submerged cultures of the Basidiomycete Tyromyces floriformis to the medium composition

机译：乳酸髓质培养物中筛选培养物的反应筛选到培养基组成

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Tyromyces floriformis, a potent fungal sesquiterpene producer, was grown Cerrena unicolor, as a model organism in submerged culture to search for chemicals affecting sesquiterpene biosynthesis in vitro. Thirty-one sesquiterpenes and sesquiterpenoids were identified in the supernatant, among them the fruity ?-ylangene as the main volatile. Additives, such as some polysaccharides or lipids, did not affect the qualitative product spectrum but strongly affected the quantitative synthesis. Rye arabinoxylan and other polysaccharides, such as chitin, starch, and agarose, almost blocked the synthesis of ?-ylangene. Single addition of the building blocks of arabinoxylan, arabinose, xylose, or ferulic acid showed no inhibitory effect, whereas 0.05% (w/v) 3(2)-?-l-arabinofuranosyl-xylobiose and larger oligosaccharides resulted in a significant suppression. In contrast, addition of acetyl donors boosted the ?-ylangene concentration by 1 order of magnitude up to >40 mg L-?1. Both increased as well as decreased ?-ylangene concentrations correlated with the intracellular sesquiterpene cyclase activity. Similar experiments using submerged cultured Cerrena unicolor, Postia placenta, and Coprinopsis cinerea showed that the additives affected fungal sesquiterpenoid synthesis differently. Whereas the addition of acetyl donors boosted the synthesis in all biphasic cultures, it was inhibited by polysaccharides in fungi preferably interacting with lignified plants. In contrast, Cerrena unicolor, known for a symbiotic lifestyle with wasps, responded by forming higher concentrations of the possibly insect-attracting sesquiterpenes.
机译：一种强大的真菌SesquiterPene生产商罗米菌弗洛米特（Floriformis）是生长的Cerena Unicor，作为淹没文化的模型生物，以寻找体外影响癫痫患者的化学品。在上清液中鉴定在上清液中的三十一体粒子荚膜，其中果味α-亚胺作为主要挥发性。添加剂，例如一些多糖或脂质，并不影响定性产品光谱，但强烈影响定量合成。黑麦阿拉伯辛和其他多糖，如甲壳素，淀粉和琼脂糖，几乎阻塞了α-番烯的合成。单一添加阿拉伯辛烷基，阿拉伯糖，木糖或阿魏酸的构建块，显示出没有抑制作用，而0.05％（w / v）3（2） - α - α - α - α - γ-r-阿拉伯呋喃糖基 - 吡糖和较大的寡糖导致显着的抑制。相反，加入乙酰基供体将α-亚胺浓度提高1级，高达> 40mg L-1-α1。随着与细胞内均质萜烯环酶活性相关的，两者均增加以及泛壬烯浓度增加。使用浸没式培养的Cerrena Unicolog，Postia胎盘和Coprinopsis Cinerea的类似实验表明，添加剂影响了真菌癫痫萜类合成的不同。虽然加入乙酰基供体在所有双相培养物中升压了合成，但在真菌中的多糖抑制了它，优选与氧化植物相互作用。相比之下，Cerrena Unicolog，以黄蜂为单位生活方式而闻名，通过形成更高的可能昆虫吸引的Sesquiterpenes来作出反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号