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Assessment of bacteria and archaea in metalworking fluids using massive parallel 16S rRNA gene tag sequencing

机译：使用大规模平行16S rRNA基因标签测序评估金属加工液中的细菌和古痤疮的评估

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Determination of the bacterial diversity in industry-based liquid in-use water-miscible metalworking fluid (MWF) samples was targeted by massive parallel multiplex DNA sequencing, either directly or upon pretreatment with propidium monoazide (PMA) that allows differentiation between intact and physically damaged cells. As MWFs provide a suitable basis of life for micro-organisms, the majority is preserved by biocides. Bio-concept' fluids on the other hand are bactericide free, which intentionally leads to substantial bacterial populations. Samples from both fluid types were chosen: A median of 51 operational taxonomic units at genera level (OTUs) were detected per sample, but only 13 were present at or above 1.0% of the total population in any PMA-treated sample analysed. As both fluid types were mainly dominated by Pseudomonas spp., we resolved this genus on the species level and found the Pseudomonas oleovorans/pseudoalcaligenes group to predominate. We also looked for archaea and detected Methanobrevibacter spp., albeit in 3% of all samples analysed.
机译：基于工业的液体中使用的水混溶性金属加工液（MWF）样品的细菌多样性的测定通过大规模的并联多重DNA测序，直接或用单氮化物（PMA）进行预处理，允许在完整和物理损坏之间进行分化的预处理细胞。由于MWFS为微生物提供了合适的生命基础，因此大多数由杀生物剂保存。另一方面的生物概念的液体是自由杀菌剂，其故意导致大量的细菌群体。选择来自两种流体类型的样品：每个样品检测到属于51个运营分类单位的中值（OTUS），但在分析任何PMA处理的样品中只有13个或高于1.0％的群体。由于两种流体类型主要由Pseudomonas SPP主导。，我们解决了物种水平的这种属，并发现伪霉素Oleovorans / Pseudoalcaligenes组占主导地位。我们还寻找archaea并检测到甲烷杆菌SPP。，尽管如此分析所有样品的3％。

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