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首页> 外文期刊>Nature cell biology >Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis
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Dynamic subunit turnover in ESCRT-III assemblies is regulated by Vps4 to mediate membrane remodelling during cytokinesis

机译：ESCRT-III组件中的动态亚基转换由VPS4调节，以在细胞因子期间介导膜重塑

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摘要

The endosomal sorting complex required for transport (ESCRT)-III mediates membrane fission in fundamental cellular processes, including cytokinesis. ESCRT-III is thought to form persistent filaments that over time increase their curvature to constrict membranes. Unexpectedly, we found that ESCRT-III at the midbody of human cells rapidly turns over subunits with cytoplasmic pools while gradually forming larger assemblies. ESCRT-III turnover depended on the ATPase VPS4, which accumulated at the midbody simultaneously with ESCRT-III subunits, and was required for assembly of functional ESCRT-III structures. In vitro, the Vps2/Vps24 subunits of ESCRT-III formed side-by-side filaments with Snf7 and inhibited further polymerization, but the growth inhibition was alleviated by the addition of Vps4 and ATP. High-speed atomic force microscopy further revealed highly dynamic arrays of growing and shrinking ESCRT-III spirals in the presence of Vps4. Continuous ESCRT-III remodelling by subunit turnover might facilitate shape adaptions to variable membrane geometries, with broad implications for diverse cellular processes.
机译：转运所需的内体分选复合物（ESCRT）-III在基本细胞过程中介导膜裂缝，包括细胞因子。 Escrt-III被认为形成持续的长丝，随着时间的推移，随着时间的推移增加曲率以限制膜。出乎意料的是，我们发现在人体细胞中间人的Escrt-III迅速转过亚基，同时逐渐形成较大的组件。 ESCRT-III转换依赖于ATP酶VPS4，其与ESCRT-III亚基同时积聚在中间体，并且需要组装功能性ESCRT-III结构。体外，ESCRT-III的VPS2 / VPS24亚基用SNF7并排形成并抑制进一步的聚合，但通过添加VPS4和ATP来缓解生长抑制。高速原子力显微镜进一步揭示了在VPS4存在下生长和缩小Escrt-III螺旋的高度动态阵列。亚基转交的连续ESCRT-III重塑可能促进可变膜几何形状的形状，具有广泛的影响。

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