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首页> 外文期刊>Mycotoxin Research >Effect of UV irradiation on aflatoxin reduction: a cytotoxicity evaluation study using human hepatoma cell line
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Effect of UV irradiation on aflatoxin reduction: a cytotoxicity evaluation study using human hepatoma cell line

机译：紫外线辐照对黄曲霉毒素减少的影响：使用人肝细胞系的细胞毒性评价研究

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摘要

In this proof-of-concept study, the efficacy of a medium-pressure UV (MPUV) lamp source to reduce the concentrations of aflatoxin B-1, aflatoxin B-2, and aflatoxin G(1) (AFB(1,) AFB(2), and AFG(1)) in pure water is investigated. Irradiation experiments were conducted using a collimated beam system operating between 200 to 360 nm. The optical absorbance of the solution and the irradiance of the lamp are considered in calculating the average fluence rate. Based on these factors, the UV dose was quantified as a product of average fluence rate and treatment time. Known concentrations of aflatoxins were spiked in water and irradiated at UV doses ranging from 0, 1.22, 2.44, 3.66, and 4.88 J cm(-2). The concentration of aflatoxins was determined by HPLC with fluorescence detection. LC-MS/MS product ion scans were used to identify and semi-quantify degraded products of AFB(1), AFB(2), and AFG(1). It was observed that UV irradiation significantly reduced aflatoxins in pure water (p 0.05). Irradiation doses of 4.88 J cm(-2) reduced concentrations 67.22% for AFG(1), 29.77% for AFB(2), and 98.25% for AFB(1) (p 0.05). Using this technique, an overall reduction of total aflatoxin content of approximate to 95% (p 0.05) was achieved. We hypothesize that the formation of OH radicals initiated by UV light may have caused photolysis of AFB(1), AFB(2), and AFG(1) molecules. In cell culture studies, our results demonstrated that the increase of UV dosage decreased the aflatoxin-induced cytotoxicity in HepG(2) cells. Therefore, our research finding suggests that UV irradiation can be used as an effective technique for the reduction of aflatoxins.
机译：在该概念证明研究中，中压UV（MPUV）灯源的功效降低了黄曲霉毒素B-1，黄曲霉毒素B-2和黄曲霉毒素G（1）（AFB（1，）AFB的浓度（2），并研究了纯水中的AFG（1））。使用200至360nm的准直梁系统进行辐射实验。溶液的光学吸收和灯的辐照度被认为是计算平均注量速率。基于这些因素，UV剂量被定量为平均流量率和治疗时间的产物。已知的黄曲霉毒素的浓度在水中掺入并以0,1.22,2.44,3.66和4.88J厘米（-2）的UV剂量照射。通过HPLC测定黄曲霉毒素的浓度，具有荧光检测。 LC-MS / MS产品离子扫描用于鉴定和半量化AFB（1），AFB（2）和AFG（1）的降解产物。观察到紫外线辐照显着降低纯水中的黄曲霉毒素（P <0.05）。 4.88J厘米（-2）的辐照剂量为AFG（1）的浓度为67.22％，对于AFB（2）的29.77％，AFB（1）的98.25％（P <0.05）。使用该技术，实现了近似约95％（P <0.05）的总黄曲霉毒素含量的总体降低。我们假设由UV光引发的OH基团的形成可能导致AFB（1），AFB（2）和AFG（1）分子的光解。在细胞培养研究中，我们的结果表明，UV剂量的增加降低了Hepg（2）细胞中的黄曲霉毒素诱导的细胞毒性。因此，我们的研究发现表明UV辐照可以用作减少黄曲霉毒素的有效技术。

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  • 来源
    《Mycotoxin Research》 |2017年第4期|共8页
  • 作者

    Patras Ankit; Julakanti Sharath; Yannam Sudheer; Bansode Rishipal R.; Burns Mallory; Vergne Matthew J.;

  • 作者单位

    Tennessee State Univ Dept Agr &

    Environm Sci Food Biosci &

    Technol Program Nashville TN 37209 USA;

    Tennessee State Univ Dept Agr &

    Environm Sci Food Biosci &

    Technol Program Nashville TN 37209 USA;

    Tennessee State Univ Dept Agr &

    Environm Sci Food Biosci &

    Technol Program Nashville TN 37209 USA;

    North Carolina A&

    T State Univ Ctr Excellence Postharvest Technol North Carolina Res Campus Kannapolis NC USA;

    Lipscomb Univ Dept Pharmaceut Sci Nashville TN 37204 USA;

    Lipscomb Univ Dept Pharmaceut Sci Nashville TN 37204 USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 Q939.5;
  • 关键词

    Aflatoxins; UVirradiation; Toxicity; Dose;

    机译：黄曲霉毒素;UVIARRADIATION;毒性;剂量;

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