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Transsynaptic Mapping of Second-Order Taste Neurons in Flies by trans -Tango

机译：Trans-Tango的二阶味道神经元的突触突触映射

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Summary Mapping neural circuits across defined synapses is essential for understanding brain function. Here we describe trans -Tango, a technique for anterograde transsynaptic circuit tracing and manipulation. At the core of trans -Tango is a synthetic signaling pathway that is introduced into all neurons in the animal. This pathway converts receptor activation at the?cell surface into reporter expression through site-specific proteolysis. Specific labeling is achieved by presenting a tethered ligand at the synapses of genetically defined neurons, thereby activating the pathway in their postsynaptic partners and providing genetic access to these neurons. We first validated trans -Tango in the Drosophila olfactory system and then implemented it in the gustatory system, where projections beyond the first-order receptor neurons are not fully characterized. We identified putative second-order neurons within the sweet circuit that include projection neurons targeting known neuromodulation centers in the brain. These experiments establish trans -Tango as a flexible platform for transsynaptic circuit analysis. Highlights ? A genetic approach for transsynaptic tracing and manipulation of neural circuits ? Genetic access to neurons based on their connectivity ? Glomerulus-specific patterns of second-order neurons in the fly olfactory system ? Identifying second-order gustatory neurons and their target areas in the fly brain Talay and Richman et?al. develop a genetic method for transsynaptic labeling of neural circuits in Drosophila . They validate it in the olfactory system and implement it in the gustatory system to reveal second-order projections of sweet tastant-responsive neurons.
机译：摘要映射跨定突触的神经电路对于了解大脑功能至关重要。在这里，我们描述了Trans -Tango，一种用于前晶跨突触电路跟踪和操纵的技术。在Trans -Tango的核心是一种合成信号传导途径，其被引入动物中的所有神经元。该途径通过特异性蛋白水解将受体激活转化为报告表达。通过在遗传定义的神经元的突触处呈现束缚配体来实现特异性标记，从而在其后腹泻的合作伙伴中激活途径并提供对这些神经元的遗传访问。我们首先在果蝇嗅觉系统中验证了Trans -Tango，然后在肠道系统中实施，其中超出一阶受体神经元的投影没有完全表征。我们在甜水电路内识别推定的二阶神经元，其包括靶向大脑中已知的神经调节中心的投影神经元。这些实验建立了Trans -Tango作为跨突触电路分析的灵活平台。强调 ？跨突触追踪和神经电路操纵的遗传方法？基于它们的连通性对神经元的遗传进入？飞嗅系统中的二阶神经元的肾小球特异性模式？在苍蝇脑塔拉和Richman等中鉴定二阶味觉神经元及其目标区域。制定果蝇神经电路横幕标记的遗传方法。它们在嗅觉系统中验证它，并在肠系系统中实施，揭示甜味响应神经元的二阶预测。

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