Filopodia Conduct Target Selection in Cortical Neurons Using Differences in Signal Kinetics of a Single Kinase

Yu-Ting Mao; Julia X. Zhu; Kenji Hanamura; Giuliano Iurilli; Sandeep Robert Datta; Matthew B. Dalva

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Filopodia Conduct Target Selection in Cortical Neurons Using Differences in Signal Kinetics of a Single Kinase

机译：Filopodia使用单一激酶的信号动力学差异进行皮质神经元的靶选择

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Dendritic filopodia select synaptic partner axons by interviewing the cell surface of potential targets, but how filopodia decipher the complex pattern of adhesive and repulsive molecular cues to find appropriate contacts is unknown. Here, we demonstrate in cortical neurons that a single cue is sufficient for dendritic filopodia to reject or select specific axonal contacts for elaboration as synaptic sites. Super-resolution and live-cell imaging reveals that EphB2 is located in the tips of filopodia and at nascent synaptic sites. Surprisingly, a genetically encoded indicator of EphB kinase activity, unbiased classification, and a photoactivatable EphB2 reveal that simple differences in the kinetics of EphB kinase signaling at the tips of filopodia mediate the choice between retraction and synaptogenesis. This may enable individual filopodia to choose targets based on differences in the activation rate of a single tyrosine kinase, greatly simplifying the process of partner selection and suggesting a general principle.

机译：树突式Filopodia通过面试潜在目标的细胞表面选择突触伴侣轴突，但是氟化绦虫如何破译粘合剂和排斥分子提示的复杂模式是如何找到适当的触点。在这里，我们在皮质神经元中证明了单个提示足以用于树突状氟化绦虫，以拒绝或选择用于突触位点的特定轴突触点。超分辨率和活细胞成像显示EphB2位于氟覆的尖端和新生突触位点。令人惊讶的是，Ephb激酶活性的遗传编码指示剂，无偏见的分类和可光活化的EphB2揭示了Ephb激酶信号传导在Filopodia尖端中的简单差异介导收缩和突触突触之间的选择。这可能使单个氟化碳基于单个酪氨酸激酶的激活率的差异来选择靶标，大大简化了合作伙伴选择的过程并提出了一般原则。

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《Neuron》 |2018年第4期|共16页
作者
Yu-Ting Mao; Julia X. Zhu; Kenji Hanamura; Giuliano Iurilli; Sandeep Robert Datta; Matthew B. Dalva;
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作者单位

Department of Neuroscience and Jefferson Synaptic Biology Center The Vickie and Jack Farber;

Department of Neuroscience and Jefferson Synaptic Biology Center The Vickie and Jack Farber;

Department of Neuroscience and Jefferson Synaptic Biology Center The Vickie and Jack Farber;

Department of Neurobiology Harvard Medical School;

Department of Neurobiology Harvard Medical School;

Department of Neuroscience and Jefferson Synaptic Biology Center The Vickie and Jack Farber;

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原文格式 PDF
正文语种 eng
中图分类神经病学;
关键词
synaptogenesis; neuronal development; cell signaling; optogenetics; photoactivation; kinase reporter; technology development; modeling; transfectable indicator;

机译：Synaptogis;神经元发展;细胞信号传导;光学学;PhotoCtivation;激酶记者;技术开发;建模;转染指示;

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1. Filopodia Conduct Target Selection in Cortical Neurons Using Differences in Signal Kinetics of a Single Kinase [J] . Yu-Ting Mao, Julia X. Zhu, Kenji Hanamura, Neuron . 2018,第4期

机译：Filopodia使用单一激酶的信号动力学差异进行皮质神经元的靶选择
2. Differences in survival-promoting effects and intracellular signaling properties of BDNF and IGF-1 in cultured cerebral cortical neurons. [J] . Yamada M, Tanabe K, Wada K, Journal of Neurochemistry: Offical Journal of the International Society for Neurochemistry . 2001,第5期

机译：BDNF和IGF-1在培养的大脑皮层神经元中的存活促进作用和细胞内信号传导特性的差异。
3. N-methyl-D-aspartate and brain-derived neurotrophic factor induce distinct profiles of extracellular signal-regulated kinase, mitogen- and stress-activated kinase, and ribosomal s6 kinase phosphorylation in cortical neurons. [J] . Rakhit S, Clark CJ, Oshaughnessy CT, Molecular pharmacology. . 2005,第4期

机译：N-甲基-D-天门冬氨酸和脑源性神经营养因子在皮质神经元中诱导细胞外信号调节激酶，促分裂原和应激激活激酶以及核糖体s6激酶磷酸化的独特特征。
4. Novel dynamics of dendritic integration in the high conductance state of cortical neurons [C] . Michael Rudolph, Alain Destexhe Computational neuroscience meeting . 2002

机译：皮质神经元高导态树突上的新动态
5. Representation and selection of time-varying signals by single cortical neurons. [D] . Toups, Jonathan Vincent. 2009

机译：单个皮质神经元表示和选择时变信号。
6. Filopodia Conduct Target Selection in Cortical Neurons using Differences in Signal Kinetics of a Single Kinase [O] . Yu-Ting Mao, Julia X. Zhu, Kenji Hanamura, -1

机译：Filopodia使用单个激酶的信号动力学差异在皮质神经元中进行目标选择。
7. Faculty Opinions recommendation of Filopodia conduct target selection in cortical neurons using differences in signal kinetics of a single kinase. [O] . Stephanie Gupton 2018

机译：菲奥普奈西亚的教师意见建议在皮质神经元中进行靶选择，使用单一激酶的信号动力学差异。

Filopodia Conduct Target Selection in Cortical Neurons Using Differences in Signal Kinetics of a Single Kinase

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