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首页> 外文期刊>Nucleus >Identification of Fkh1 and Fkh2 binding site variants associated with dynamically bound DNA elements including replication origins
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Identification of Fkh1 and Fkh2 binding site variants associated with dynamically bound DNA elements including replication origins

机译：鉴定与动态结合的DNA元素相关的FKH1和FKH2结合位点变体，包括复制起源

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摘要

Forkhead Box (Fox) DNA binding proteins control multiple genome activities, including transcription, replication, and repair. These activities are organized spatially and temporally in the nucleus, and Fox proteins Fkh1 and Fkh2 have emerged as regulators of long-range chromosomal interactions involved with these activities, such as the clustering of replication origins programmed for early initiation. Fkh1 and Fkh2 bind a subset of replication origins and are thought to dimerize to mediate long-range chromosomal contacts between these origins. The binding of Fkh1 and/or Fkh2 (Fkh1/2) to replication origins and the recombination enhancer (RE), which is involved in DNA repair required for mating-type switching, is cell cycle-regulated and thus appears to be more dynamic than Fkh1/2 binding at regulated target genes. Here we report the identification of Fkh1/2 binding sequence variants at replication origins and the RE compared with Fkh1/2 binding sequences found at target genes of the CLB2 group. These different binding sequences have previously been characterized as weak and strong, respectively, suggesting that the presence of weak sites contributes to more dynamic interactions at replication origins and RE, possibly facilitated by Fkh1/2 dimerization and cooperative interactions with accessory proteins. We discuss the wealth of regulatory potential imbued in these features of the DNA and its binding proteins.
机译：FORKHEAD盒（FOX）DNA结合蛋白控制多种基因组活动，包括转录，复制和修复。这些活性在空间和时间上在核中组织，福克斯蛋白FKH1和FKH2被出现为与这些活动涉及的远程染色体相互作用的调节因子，例如复制的聚类来源为早期启动。 FKH1和FKH2结合复制起源的子集，并被认为二聚体以在这些起源之间介导的远程染色体接触。 FKH1和/或FKH2（FKH1 / 2）与复制起源和重组增强剂（RE）的结合，其参与配合式切换所需的DNA修复，是细胞周期调节，因此似乎比FKH1 / 2在受调节靶基因的结合。在这里，我们报告了复制起源中的FKH1 / 2结合序列变体的鉴定，与CLB2组的靶基因中发现的FKH1 / 2结合序列相比。这些不同的结合序列先前已经表征为弱和强，表明弱部位的存在有助于复制起源和RE的更具动态相互作用，并且可以通过与辅助蛋白的FKH1 / 2二聚化和合作相互作用促进。我们讨论了在DNA的这些特征中讨论了对DNA的这些特征和其结合蛋白质的财富。

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