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首页> 外文期刊>Lab on a chip >High-throughput fluorescence polarization immunoassay by using a portable fluorescence polarization imaging analyzer
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High-throughput fluorescence polarization immunoassay by using a portable fluorescence polarization imaging analyzer

机译：使用便携式荧光偏振成像分析仪来高通量荧光偏振免疫测定

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摘要

High-throughput fluorescence polarization immunoassays (FPIAs) for mycotoxin were conducted using a portable FP analyzer with a microdevice. Simultaneous FPIA measurements for 8 different deoxynivalenol (DON) concentrations in 12 chambers (total of 96 samples) and high-throughput FPIA measurements for single DON concentrations in more than 500 chambers were conducted. The results indicated that simultaneous FPIAs for 96 independent samples and for 500 samples were possible by FP imaging. The FP analyzer has a size of 65 cm (W 35 cm x D 15 cm x H 15 cm) and costs less than $5000. The sample volume was 1 nL. Furthermore, it is expected that sample reaction and FP detection can be automatically conducted with the analyzer by changing the microdevice and the software. Its features such as low cost and portability will contribute to on-site measurement and point-of-care testing. Additionally, the high-throughput feature will contribute to the study of molecular interactions based on FP measurements.
机译：使用具有微型微型的便携式FP分析仪进行霉菌毒素的高通量荧光偏振免疫测定（FPIAS）。进行了在12腔室中的8种不同脱氧酚（Don）浓度（总共96个样品）和高通量FPIA测量的同时进行FPIa测量，并进行500多个腔室中的单唐浓度的高通量FPIA测量。结果表明，通过FP成像，可以同时对96个独立样品和500个样品进行同时FPIAS。 FP分析仪的尺寸为65厘米（W 35厘米x d 15cm x H 15 cm），并且成本低于5000美元。样品体积为1 nL。此外，预期可以通过改变微量化和软件与分析仪一起自动地进行样品反应和FP检测。其特点如低成本和便携性将有助于现场测量和护理点测试。另外，高通量特征将有助于基于FP测量的分子相互作用的研究。

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  • 来源
    《Lab on a chip》 |2019年第15期|共8页
  • 作者

    Wakao Osamu; Satou Ken; Nakamura Ayano; Galkina Polina A.; Nishiyama Keine; Sumiyoshi Ken; Kurosawa Fumio; Maeki Masatoshi; Ishida Akihiko; Tani Hirofumi; Proskurnin Mikhail A.; Shigemura Koji; Hibara Akihide; Tokeshi Manabu;

  • 作者单位

    Hokkaido Univ Grad Sch Chem Sci &

    Engn Kita Ku Kita 13 Nishi 8 Sapporo Hokkaido 0608628 Japan;

    Tianma Japan Ltd Saiwai Ku Shin Kawasaki Mitsui Bldg West Tower 28F 1-1-2 Kawasaki Kanagawa 2120058 Japan;

    Hokkaido Univ Grad Sch Chem Sci &

    Engn Kita Ku Kita 13 Nishi 8 Sapporo Hokkaido 0608628 Japan;

    Moscow MV Lomonosov State Univ Chem Dept GSP-2 Moscow 119991 Russia;

    Hokkaido Univ Grad Sch Chem Sci &

    Engn Kita Ku Kita 13 Nishi 8 Sapporo Hokkaido 0608628 Japan;

    Tianma Japan Ltd Saiwai Ku Shin Kawasaki Mitsui Bldg West Tower 28F 1-1-2 Kawasaki Kanagawa 2120058 Japan;

    Tohoku Univ Inst Multidisciplinary Res Adv Mat Aoba Ku Katahira 2-1-1 Sendai Miyagi 9808577 Japan;

    Hokkaido Univ Fac Engn Div Appl Chem Kita Ku Kita 13 Nishi 8 Sapporo Hokkaido 0608628 Japan;

    Hokkaido Univ Fac Engn Div Appl Chem Kita Ku Kita 13 Nishi 8 Sapporo Hokkaido 0608628 Japan;

    Hokkaido Univ Fac Engn Div Appl Chem Kita Ku Kita 13 Nishi 8 Sapporo Hokkaido 0608628 Japan;

    Moscow MV Lomonosov State Univ Chem Dept GSP-2 Moscow 119991 Russia;

    Tianma Japan Ltd Saiwai Ku Shin Kawasaki Mitsui Bldg West Tower 28F 1-1-2 Kawasaki Kanagawa 2120058 Japan;

    Tohoku Univ Inst Multidisciplinary Res Adv Mat Aoba Ku Katahira 2-1-1 Sendai Miyagi 9808577 Japan;

    Hokkaido Univ Fac Engn Div Appl Chem Kita Ku Kita 13 Nishi 8 Sapporo Hokkaido 0608628 Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学实验（实验化学）;生物化学;生物科学;化学;
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