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Pneumatic microfluidic cell compression device for high-throughput study of chondrocyte mechanobiology

机译：软骨细胞机动学高通量研究的气动微流体细胞压缩装置

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摘要

Hyaline cartilage is a specialized type of connective tissue that lines many moveable joints (articular cartilage) and contributes to bone growth (growth plate cartilage). Hyaline cartilage is composed of a single cell type, the chondrocyte, which produces a unique hydrated matrix to resist compressive stress. Although compressive stress has profound effects on transcriptional networks and matrix biosynthesis in chondrocytes, mechanistic relationships between strain, signal transduction, cell metabolism, and matrix production remain superficial. Here, we describe development and validation of a polydimethylsiloxane (PDMS)-based pneumatic microfluidic cell compression device which generates multiple compression conditions in a single platform. The device contained an array of PDMS balloons of different sizes which were actuated by pressurized air, and the balloons compressed chondrocytes cells in alginate hydrogel constructs. Our characterization and testing of the device showed that the developed platform could compress chondrocytes with various magnitudes simultaneously with negligible effect on cell viability. Also, the device is compatible with live cell imaging to probe early effects of compressive stress, and it can be rapidly dismantled to facilitate molecular studies of compressive stress on transcriptional networks. Therefore, the proposed device will enhance the productivity of chondrocyte mechanobiology studies, and it can be applied to study mechanobiology of other cell types.
机译：透明软骨是一种专用类型的连接组织，可在许多可移动的关节（关节软骨）上线，并有助于骨骼生长（生长钢板软骨）。透明软骨由单个细胞类型，软骨细胞组成，其产生独特的水合基质以抵抗压缩应力。虽然压缩应力对软骨细胞的转录网络和基质生物合成具有深远的影响，但应变，信号转导，细胞代谢和基质生产之间的机械关系保持浅表。这里，我们描述了基于聚二甲基硅氧烷（PDMS）的气动微流体细胞压缩装置的开发和验证，该微流体细胞压缩装置在单个平台中产生多个压缩条件。该器件包含由加压空气驱动的不同尺寸的PDMS球囊阵列，并且气球压缩在海藻酸盐水凝胶构建体中的软骨细胞细胞。我们的表征和测试的表征和测试表明，发育平台可以同时用各种大小压制软骨细胞，对细胞活力的影响可忽略不计。此外，该装置与直播细胞成像兼容以探测压缩应力的早期影响，并且可以迅速拆除以促进对转录网络上的压缩应力的分子研究。因此，所提出的装置将增强软骨细胞机动学研究的生产率，可以应用于研究其他细胞类型的力学生物学。

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