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首页> 外文期刊>FEBS letters. >Arabidopsis Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth
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Arabidopsis Arabidopsis pollen extensins LRX are required for cell wall integrity during pollen tube growth

机译:在花粉管生长期间,拟南芥Arabidopsis花粉extensins LRX是细胞壁完整性所必需的

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摘要

Proper cell wall assembly is crucial during pollen tube growth. Leucine‐rich repeat extensins (LRXs) are extracellular glycoproteins which belong to the hydroxyproline‐rich glycoprotein (HRGP) family. They contain a conserved N‐terminal leucine‐rich repeat (LRR) domain and a highly variable C‐terminal extensin domain. Here, we characterized four LRX proteins (LRX8 through LRX11) from pollen of Arabidopsis thaliana . To investigate the role of LRX8‐LRX11 in pollen germination and pollen tube growth, multiple T‐DNA lrx mutants were obtained. The lrx mutants display abnormal pollen tubes with an irregular deposition of callose and pectin. They also show serious alterations in pollen germination and segregation ratio. Our results suggest that LRXs are involved in ensuring proper cell wall assembly during pollen tube growth.
机译:适当的细胞壁组件在花粉管生长期间至关重要。 富含亮氨酸的重复延长素(LRX)是属于富含富羟脯氨酸的糖蛋白(HRGP)家族的细胞外糖蛋白。 它们含有保守的N-末端亮氨酸富氨氨酸的重复(LRR)域和高度可变的C末端延伸域。 在这里,我们从拟南芥的花粉中表征了四种LRX蛋白(LRX8至LRX11)。 为了探讨LRX8-LRX11在花粉萌发和花粉管生长中的作用,得到多种T-DNA LRX突变体。 LRX突变体显示出对胼舌和果胶的不规则沉积的异常花粉管。 他们还显示出花粉萌发和隔离比例的严重改变。 我们的研究结果表明,LRX在花粉管生长期间涉及确保适当的细胞壁组装。

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