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首页> 外文期刊>Nitric oxide: Biology and chemistry >Saliva panel of protein candidates: A comprehensive study for assessing high altitude acclimatization
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Saliva panel of protein candidates: A comprehensive study for assessing high altitude acclimatization

机译：唾液蛋白候选者：评估高海拔适应症的综合研究

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Altitude acclimatization describes the processes whereby lowland humans respond to decreased partial pressure of oxygen. It refers to the changes seen as beneficial and involves a series of physiological adjustments that compensate for reduced ambient PO2, as opposed to changes that are pathological. Although numerous reports document the physiological effects of exposure to hypobaric hypoxia of varying durations but an interesting aspect overlooked by many researchers is that of acclimatization related studies. As proteome, a dynamic entity responds immediately to external stimuli, protein markers and their trends can be studied to assess acclimatization status of an individual. Compared to blood, the use of saliva is advantageous because sample collection and processing are easy, minimally invasive, low cost and better tolerated by individuals. In this study, we employed iTRAQ based LC-MS/MS technique for comparing saliva samples from humans exposed to hypobaric hypoxia from 7 to 120 days with normoxic controls followed by analysis using Ingenuity Pathway Analysis software and validation by immunoassays. Nearly 67 proteins were found to be differentially expressed in the exposed groups as compared to normoxia indicating modulated canonical pathways as lipid metabolism; acute phase response signalling and proteins as carbonic anhydrase 6, alpha-enolase, albumin, and prolactin inducible protein. Collectively, this study provides the proof of concept for the non-invasive assessment of high altitude acclimatization.
机译：高度适应性化描述了低地人类对氧气部分压力降低的过程。它是指视为有益的变化，涉及一系列补偿减少的环境PO2的生理调整，而不是病态的变化。虽然许多报告记录了暴露于不同持续时间的低次缺氧的生理效应，但许多研究人员忽视的有趣方面是适应相关的相关研究。作为蛋白质组，动态实体立即应对外部刺激，蛋白质标记及其趋势可以研究以评估个人的适应状态。与血液相比，唾液的使用是有利的，因为样品收集和加工容易，最小的侵入性，低成本，并且由个体更好地耐受。在这项研究中，我们使用基于ITRAQ的LC-MS / MS技术，用于将唾液样本与常氧对照中的7-120天暴露于低管缺氧，然后使用ImmunoAssays的验证分析和验证。与指示脂质代谢作为脂质代谢的调节典型途径相比，发现近67个蛋白质在暴露基团中差异表达;急性相响应信号和蛋白质作为碳酸酐酶6，α-烯醇酶，白蛋白和催乳素诱导蛋白。本研究统称，为高海拔适应化的非侵入性评估提供了概念证明。

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