• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Tropical Animal Health and Production >A panel of protein candidates for comprehensive study of Caprine Arthritis Encephalitis (CAE) infection
【24h】

A panel of protein candidates for comprehensive study of Caprine Arthritis Encephalitis (CAE) infection

机译：综合素候选综合研究，用于综合研究河口关节炎脑炎（CAE）感染

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The caprine arthrite encephalite (CAE) is a disease that affects especially dairy goat. The virus shows compartmentalization features, that allows it to hide at certain times during the course of the disease, making it difficult to control. The present study was conducted to identify the major seminal plasma protein profile of goats infected by CAE and its associations with seroconversion using Western blotting. Two groups containing five males each, were used in this experiment. The first group was composed by seropositive animals and the control by seronegative confirmed by Western blotting and PCR. The semen was collected through artificial vagina and after that, two-dimensional electrophoresis and MALDI-TOF MS were used. Seventy-five spots were identified in the goat seminal plasma gels, equivalent to 13 different proteins with more expression. The similar proteins found in both groups and related to reproduction were spermadhesin Z13-like, bodhesin and bodhesin-2, Lipocalin, protein PDC-109-like, and albumin. In infected goats, proteases such as arisulfatase A have been identified, whose function probably is related to metabolism control of sulfatides, involved to virus control. The other ones were bifunctional ATP-dependent dihydroxyacetone kinase/FAD-AMP lyase, cathepsin F isoform X1, disintegrin and metalloproteinase domain-containing protein 2-like isoform X1, clusterin, carbonic anhydrase 2, electron transfer flavoprotein subunit beta, and epididymal secretory glutathione peroxidase. The results of this study show the reaction of the innate immune system against chronic infection of goats by CAE.
机译：Caprine关节分子脑骨髓（CAE）是一种影响尤其是乳制品山羊的疾病。病毒显示分区化特征，使其在疾病过程中的某些时间内允许其隐藏，使得难以控制。进行了本研究以鉴定CAE感染的山羊的主要精血浆蛋白质概况及其与血清转换使用Western印迹。在本实验中使用了两组含有五个男性的群体。第一组由血清阳性动物组成，并通过Western Blotting和PCR证实的血清基因的对照组成。通过人工阴道收集精液，之后，使用二维电泳和MALDI-TOF MS。在山羊半血浆凝胶中鉴定了七十五斑，相当于具有更多的表达的13种不同的蛋白质。在两组中发现的类似蛋白质和与繁殖相关的蛋白质是生物蛋白Z13样，肌醇蛋白和肌腱蛋白-2，Lipocalin，蛋白质PDC-109样和白蛋白。在感染的山羊中，已经鉴定了诸如arisulfat酶A的蛋白酶，其功能可能与硫酸肽的代谢控制有关，涉及病毒对照。另一个是双官能ATP依赖性二羟基丙酮激酶/ FAD-AMP裂解酶，组织蛋白酶F同食X1，Disintegl和金属蛋白酶畴样同种型X1，簇蛋白，碳酸酐酶2，电子转移黄蛋白亚基β，和附睾分泌谷胱甘肽过氧化物酶。该研究的结果显示了先天免疫系统对CAE的山羊慢性感染的反应。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号