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首页> 外文期刊>LWT-Food Science & Technology >Liposome encapsulation of anionic and cationic whey peptides: Influence of peptide net charge on properties of the nanovesicles
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Liposome encapsulation of anionic and cationic whey peptides: Influence of peptide net charge on properties of the nanovesicles

机译：阴离子和阳离子乳清肽的脂质体包封：肽净电荷对纳米粒子性质的影响

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摘要

Poor bioavailability of food-derived bioactive peptides impedes their application in functional foods. This study focuses on understanding the impact of net charge of whey peptides, separated by electrodialysis with ultrafiltration membrane (EDUF), on encapsulation efficiency (EE) and surface properties of the peptide-loaded liposomes. EDUF resulted in two fractions; anionic whey peptides (AWP) with a relative abundance of 30.4% anionic amino acid residues, and cationic whey peptides (CWP) with 20.39% cationic amino acid residues. Although free amino group of CWP was higher, average peptide length and molecular weight were similar in both fractions. AWP liposomes had slightly lower encapsulation efficiency than encapsulated CWP. Encapsulated CWP had larger mean particle diameter and higher polydispersity index. However, there was no apparent effect of peptide net charge on liposome surface charge zeta-potential). MR spectra of the CWP- and AWP-loaded liposomes were similar and showed peptide interaction with the liposome phosphate groups. The peptide net charge did not appear to influence their distribution in different regions of the nanocapsules. The EE and physicochemical properties of the liposomes may be influenced by a combination of peptide net charge, homogeneity, and surface hydrophobicity. These findings are relevant to developing efficient liposomal systems for bioactive peptide delivery. (C) 2017 Elsevier Ltd. All rights reserved.
机译：食物衍生的生物活性肽的生物利用度差阻碍了它们在功能性食物中的应用。本研究重点介绍了解乳清肽净电荷的影响，用电渗析与超滤膜（EDUF）分离，对肽加载脂质体的封装效率（EE）和表面性质。 EDUF导致两分数;阴离子乳清肽（AWP）具有相对丰富的30.4％阴离子氨基酸残基，阳离子乳清肽（CWP），具有20.39％的阳离子氨基酸残基。虽然CWP的游离氨基较高，但两种级分中平均肽长度和分子量相似。 AWP脂质体的封装效率略低于封装的CWP。封装的CWP具有较大的平均粒径和更高的多分散指数。然而，肽净电荷对脂质体表面电荷ζ电位没有明显的影响。 CWP和AWP加载的脂质体的MR光谱相似，并显示肽与脂质体磷酸基团相互作用。肽净电荷似乎没有影响其在纳米胶囊的不同区域中的分布。脂质体的EE和物理化学性质可以受肽净电荷，均匀性和表面疏水性的组合的影响。这些发现与显影有效的生物活性肽递送的脂质体系统有关。 （c）2017 Elsevier Ltd.保留所有权利。

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