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首页> 外文期刊>Limnology >Establishing an environmental DNA method to detect and estimate the biomass of Sakhalin taimen, a critically endangered Asian salmonid
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Establishing an environmental DNA method to detect and estimate the biomass of Sakhalin taimen, a critically endangered Asian salmonid

机译：建立环境DNA方法以检测和估算Sakhalin Taimen的生物量，危及濒危亚洲鲑鱼

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摘要

For field ecologists, detecting a target species in the wild is a severe bottleneck to understanding its distribution and population status. Recently, environmental DNA (eDNA) techniques have been developed as a noninvasive monitoring tool for aquatic organisms. While applications of eDNA techniques for biomass estimation have been proposed, little is known about an applicable size range of the organisms, which might affect relationships between biomass and eDNA concentration. Here, we investigated eDNA from Sakhalin taimen (Parahucho perryi), a giant salmonid species of northern Japan. This species is critically endangered and difficult to detect in the wild by conventional sampling methods. Using quantitative real-time PCR, we tested correlations between eDNA concentration and fish density using fish with a wide range of ages and body sizes in aquarium experiments. We found that our new primers and probe were truly species-specific, and that the eDNA concentration was significantly correlated with fish density and body size (p 0.001). Furthermore, based on our calculation, the eDNA concentrations were rather constant among aquaria with fish in different age and size groups when their total weight was adjusted. These results suggest that eDNA concentrations can be an indicator of biomass of Sakhalin taimen, although further research is needed for its application in natural environments.
机译：对于野外生态学家，检测野外的目标物种是理解其分布和人口地位的严重瓶颈。最近，已经开发了环境DNA（EDNA）技术作为水生生物的非侵入性监测工具。虽然已经提出了对生物量估计的EDNA技术的应用，但是关于有机体的适用尺寸范围，这可能影响生物量和EDNA浓度之间的关系。在这里，我们研究了来自日本北部的巨型鲑鱼类萨哈林泰汶（Parahucho Perryi）的edna。这种物种受到常规取样方法的克里危害且难以检测野外。使用定量实时PCR，我们在水族馆实验中使用具有各种年龄和身体尺寸的鱼类的eDNA浓度和鱼密度之间的相关性。我们发现我们的新引物和探针是特异性的特异性，并且EDNA浓度与鱼密度和体尺寸显着相关（P <0.001）。此外，基于我们的计算，当调整总重量时，eDNA浓度在不同年龄和大小群中的鱼类中的水族中均为恒定。这些结果表明EDNA浓度可以是Sakhalin Taimen的生物量的指标，但在自然环境中的应用需要进一步研究。

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