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Bacterial 16S rDNA and alkaline phosphatase gene diversity in soil applied with composted aquatic plants

机译:用堆肥水产植物施用土壤中的细菌16S rDNA和碱性磷酸酶基因多样性

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摘要

The present study aimed to examine the effects of applying composted aquatic plants (CAP) on richness and composition of soil bacterial assemblages responsible for alkaline phosphatase production by pot experiments, as compared to the corresponding effects of inorganic chemical fertilizers (ICF). Soil bacterial assemblages were studied by metabarcoding the 16S region of ribosomal DNA as a universal genetic marker and thephoDalkaline phosphatase gene as a functional marker. The compositions of 16S andphoDassemblages were significantly different among soils applied with CAP, ICF, their mixture (MIX), and control (no application). Furthermore, the richness ofphoDassemblages in soils was greater in CAP or MIX application than in ICF or control, although significant difference only existed between MIX and ICF. In conclusion, the present study illustrated that the application of composted aquatic plants affected the richness and composition of soil bacterial assemblages responsible for the production of alkaline phosphatase.
机译:本研究旨在探讨应用堆肥水生植物(帽)对负责罐实验负责碱性磷酸酶生产的土壤细菌组合的丰富性和组成的影响,与无机化肥(ICF)的相应效果相比。通过将核糖体DNA的16S区作为普遍遗传标记物和作为官能标记物作为官能标记物来研究土壤细菌组合。在用帽,ICF,它们的混合物(混合物)和对照(无施用)施用的土壤中,16S和双散置的组合物显着不同。此外,在帽或混合应用中,在含量或对照中的含量较高,但在ICF或控制中,虽然仅存在显着差异和ICF之间的显着差异。总之,本研究表明,堆肥水生植物的应用影响了负责生产碱性磷酸酶的土壤细菌组合的丰富性和组成。

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