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首页> 外文期刊>BJU international >The expression of PAX5 in human transitional cell carcinoma of the bladder: relationship with de-differentiation.
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The expression of PAX5 in human transitional cell carcinoma of the bladder: relationship with de-differentiation.

机译：PAX5在膀胱移行细胞癌中的表达：与去分化的关系。

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OBJECTIVE: To investigate the expression of PAX genes, a family of developmental control genes (which encode nine nuclear transcription factors essential for embryogenesis and are proto-oncogenes in mice) in human transitional cell carcinoma (TCC) of the bladder. MATERIALS AND METHODS: PAX gene expression was assessed in three established bladder cancer cell lines and 29 primary tumours using the reverse transcriptase-polymerase chain reaction and Southern analysis. RESULTS: All three established TCC cell lines and 79% of primary TCCs expressed PAX5 mRNA. There was a significantly higher proportion of PAX5 expression in malignant than in benign urothelium (P=0.02, Fisher's exact test); nine of 12 pTa tumours (mucosa-confined), seven of eight pT1 (invading lamina propria) and eight of nine pT2 (invading muscle) expressed PAX5. A higher proportion of tumours with increasing de-differentiation expressed PAX5, which correlates well with the expression pattern of PAX5 in development. In well-differentiated tumours (grade 1), half expressed PAX5, compared with 84% of moderately to poorly differentiated tumours (grades 2/3). The odds ratio for PAX5 expression in malignancy suggests that it increases the risk of malignancy four-fold. CONCLUSION: These data support a role for the PAX family in oncogenesis, by identifying another human neoplasm in which they are inappropriately expressed. PAX5 expression in undifferentiated TCC cells may contribute to pathogenesis by supporting cellular proliferation in the de-differentiated state. Furthermore, the high incidence of PAX5 expression suggests its potential use as a diagnostic tool and therapeutic target in TCC.
机译：目的：研究PAX基因在膀胱移行细胞癌（TCC）中的表达。PAX基因是一个发育控制基因家族（编码9个核转录因子，是胚胎发生所必需的，并且是小鼠的原癌基因）。材料与方法：使用逆转录酶-聚合酶链反应和Southern分析评估了3种已建立的膀胱癌细胞系和29种原发性肿瘤中PAX基因的表达。结果：所有三个已建立的TCC细胞系和79％的原发性TCC表达PAX5 mRNA。恶性肿瘤中PAX5表达的比例明显高于良性尿路上皮（P = 0.02，Fisher精确检验）。 12个pTa肿瘤中有9个（粘膜受限），8个pT1中的7个（固有层侵犯）和9个pT2中的8个（侵犯肌肉）表达了PAX5。越来越多的去分化增加的肿瘤表达了PAX5，这与PAX5在发育中的表达模式密切相关。在高度分化的肿瘤（1级）中，一半表达PAX5，而中度至低分化的肿瘤（2/3级）则为84％。 PAX5在恶性肿瘤中表达的优势比表明，​​它使恶性肿瘤的风险增加了四倍。结论：这些数据通过鉴定另一种不适当表达的人类肿瘤，支持了PAX家族在肿瘤发生中的作用。未分化的TCC细胞中PAX5的表达可能通过支持去分化状态下的细胞增殖来促进发病机理。此外，PAX5表达的高发生率表明其可能用作TCC中的诊断工具和治疗靶标。

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