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首页> 外文期刊>Research journal of pharmacy and technology >Pharmacognostic Profile of Trigonella comiculata L. Seeds and effect of its Aqueous Extract on Growth Inhibition of Cancer Cells
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Pharmacognostic Profile of Trigonella comiculata L. Seeds and effect of its Aqueous Extract on Growth Inhibition of Cancer Cells

机译：Trigonella Comiculata L.种子和水提取物对癌细胞生长抑制的疗效的药物查找

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摘要

Trigonella comiculata L. (kasuri methi) is used as a pot herb especially in Northern parts of India. The seeds of the plant hold immense therapeutic potential but, the reports on its pharmacognostic validation are not available. Thus, the present investigation provides a detailed description of pharmacognostical characteristics viz., macroscopic and microscopic features, ash values, extractive values, fluorescence behavior and preliminary estimation of phytochemicals in the seeds. Macroscopic analysis showed elliptical shaped seeds with dimensions 1.5x1.0x0.15 (length, breadth and thickness). The microscopic transverse section of seeds showed two cotyledons enclosed in a mucilaginous endosperm with semilunar radicle. The powder microscopy showed the presence of epidermal and palisade cells of testa, aleurone grains and oil globules. The seeds exhibited characteristic fluorescence in response to reaction with different chemical reagents. The presence of alkaloids, phenolic compounds, flavonoids, carbohydrates, proteins, amino acids and phytosterols were recorded in preliminary phytochemical screening. To the best of our knowledge, the present study establishes the first pharmacognostic documentation of Trigonella comiculata L. seed to validate its authentication and purity. Furthermore, the aqueous seed extract was prepared and evaluated for its effect on cell lines viability of five malignant (MG-63, IMR-32, HeLa, A549, HepG2) and one non malignant (293T) cells of human origin. Among all human cancer cell lines, it was observed that seed extract inhibited the growth of the malignant cells with the best activity against HepG2 cells with IC50 of 232.80 fig/ml. The extract was found to be ineffective against non-malignant 293T cells with very high IC50 of 4.10xl013 ug/ml. Thus, the seed extract showed the therapeutic potential against cancer cells which can be exploited in further investigation against carcinogenesis.
机译：Trigonella Comiculata L.（卡西利Methi）用作盆栽药草，特别是在印度北部。植物的种子持有巨大的治疗潜力，但是，还没有提供关于其药物核检验证的报告。因此，本研究提供了对药物侦查特征的详细描述。，宏观和微观特征，灰度值，提取值，荧光行为和种子中植物化学的初步估计。宏观分析显示椭圆形种子，尺寸为1.5x1.0x0.15（长度，宽度和厚度）。种子的微观横截面显示出封闭在具有半不甲状腺胚胎中的粘膜胚乳中的两个子叶。粉末显微镜表现出睾丸，葡萄酮谷物和油球的表皮和拨塔细胞存在。响应于与不同化学试剂的反应，种子表现出特征荧光。在初步的植物化学筛查中记录了生物碱，酚类化合物，类黄酮，碳水化合物，碳水化合物，蛋白质，氨基酸和植物甾醇。据我们所知，本研究建立了Trigonella Comiculata L.种子的第一个药物查询文件，以验证其认证和纯度。此外，制备水性种子提取物并评估其对5个恶性（Mg-63，IMR-32，Hela，A549，HepG2）和人来源的一个非恶性（293T）细胞的细胞系活力的影响。在所有人类癌细胞系中，观察到种子提取物抑制恶性细胞的生长，并具有232.80毫效率的IC50的HepG2细胞的最佳活性。发现提取物对非恶性293T细胞无效，其具有4.10xl013 ug / ml非常高的IC50。因此，种子提取物显示出对癌细胞的治疗潜力，其可以在进一步调查癌症中进行剥夺。

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