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首页> 外文期刊>Russian journal of genetics >ITS1-5.8S rDNA-ITS2 and trnL-trnF Sequences as Markers for the Study of Species Diversity of Altai Feather Grasses
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ITS1-5.8S rDNA-ITS2 and trnL-trnF Sequences as Markers for the Study of Species Diversity of Altai Feather Grasses

机译：其1-5.8s rdna-its2和trnl-trnf序列作为Altai羽毛草物种多样性研究的标志物

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摘要

The ITS1-5.8S rDNA-ITS2 sequence of the 35S rRNA genes of 16 species of feather grasses and 2 species of false needlegrasses of the flora of the Altai Republic and Altai krai (Stipabaicalensis, S. borysthenica, S. capillata, S. consanguinea, S. dasyphylla, S. desertorum, S. glareosa, S. grandis, S. korshinskyi, S. krylovii, S. lessingiana, S. orientalis,S. pennata, S. praecapillata, S. pulcherrima, S. zalesskii, Ptilagrostisjunatovii, and P. mongholica), as well as four feather grass species from other regions of Russia (S. pontica, S. rubens, S. tirsa, and S. ucrainica), was sequenced. The trnL-trnF chloroplast sequences of S. capillata, S. borysthenica, S. glareosa, S. krylovii, S. lessingiana, S. orientalis, S. pulcherrima, and S. zalesskii were also determined. The trnL-trnF region, as well as the 5.8S rDNA, is highly conserved in feather grasses and cannot be used to differentiate sections and species within the genus. The ITS1 and ITS2 sequences, on the contrary, have some parsimony-informative substitutions and indels, which makes it possible to identify groups of ribotypes corresponding to sections and individual species within some sections. The clades corresponding to the Stipa and Leiostipa sections were separated; the species of the Barbatae and Subbarbatae sections composed the same clade, while the section Hemibarbatae turned out to be polyphyletic. Only in the section Stipa were no species-specific nucleotide substitutions and indels revealed. The isolation of the species S. desertorum, the taxonomic rank of which was previously unclear, was shown. The origin of polymorphic sites in the ITS sequences of feather grasses is discussed as possible evidence of distant hybridization.
机译：ita1-5.8s rdna-its2的16种羽毛草的35s rRNA基因的序列和阿尔泰共和国和阿尔泰·卡莱植物区群的2种假切网豆（Stipabaicalensis，S.Borysthenica，S. Capillata，S.Cenguinea ，S. dasyphylla，S. deserorum，S. grantosa，S. Korshinskyi，S. Krylovii，S. Lisepiana，S. Orientalis，S. Pennata，S.Praecapillata，S.Plecrima，S. Zalesskii，PtilagrostisJunatovii和P. Monghelica）以及来自俄罗斯其他地区的四种羽毛草地（S.Pontica，S. Rubens，S.Tirsa和S. ucrainica）进行测序。还确定了S.Borysthenica，S.Borysthenica，S.Grareosa，S.Krylovii，S. Lissingiana，S. Orientalis，S. pulcherrima和S. Zalesskii的Trnl-Trnf叶绿体序列。 TRNL-TRNF区域以及5.8S rDNA在羽毛草中高度保守，不能用于区分属植物内的部分和物种。相反，其1和ITS2序列具有一些关于信息的替代品和诱导，这使得可以识别与某些部分内的部分和个体物种相对应的核髓型组。分离对应于STIPA和Leiostipa部分的片状; BARBATAE和子禁止部分组成了相同的疏水板，而血蓟巴巴的截面术语是多骨。只有在STIPA部分中，STIPA没有特异性核苷酸取代，揭示。显示了物种S. Desertorum的分离，目前尚不清楚的分类级别。将羽毛曲线序列中多态性位点的起源作为遥远的杂交的可能证据讨论。

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