...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Orthopaedic surgery >Osteogenic Ability Detection of Human Bone Morphogenetic Protein-2 Gene-activated Nano Bone Putty by Reusable Double-Cavity Bone Harvest Chamber
【24h】

Osteogenic Ability Detection of Human Bone Morphogenetic Protein-2 Gene-activated Nano Bone Putty by Reusable Double-Cavity Bone Harvest Chamber

机译：通过可重复使用的双腔骨收获室，人骨形态发生蛋白-2基因活性纳米骨腻子的成骨化能力检测

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Objective: To explore the feasibility of implanting a self-designed reusable double-cavity bone harvest chamber into Guizhou mini-pigs for observation of the osteogenic effect of human bone morphogenetic protein-2 (hBMP-2) gene-activated nano bone putty on bone in growth. Methods: Eight healthy 12-month-old female Guizhou mini-pigs were used for the present experiment. In the first operation, empty double-cavity bone harvest chambers (n = 8) were implanted into the femoral metaphysis of the animals as a blank control group. In the second operation, the femoral metaphyses were implanted with the chambers filled by the nano bone putty+hBMP-2 plasmid in one cavity and nothing in the other cavity, respectively (experiment group, n = 8). The time interval between every operation was 3 months. The cavity materials were retrieved and replaced for assessment by gross observation, histological examination, and bone morphology metrology analysis to compare oste-ogenesis ability and alkaline phosphatase. Results: Three months after surgery, the nano bone putty+hBMP-2 plasmid in one cavity of the chambers had hard gray and white tissues inside, while the cavities pre-installed with nothing were filled with soft brown tissues. Light microscopy showed new generated bone tissue around the filled material, but only fibrous tissues in the empty cavities. Osteogenesis ability and alkaline phosphatase of the nano bone putty+hBMP-2 plasmid group were significantly higher than those of the blank control group (P < 0.05). Conclusion: The reusable double-cavity bone harvest chamber can be used to observe the osteogenic potential of the hBMP-2 gene-activated nano bone putty.
机译：目的：探讨将自行设计可重复使用的双腔骨收获室植入贵州迷你猪的可行性，以观察人骨形态发生蛋白-2（HBMP-2）基因活化纳米骨筋在骨骼上的成骨作用在增长中。方法：八八个健康的12个月古州迷你猪用于本实验。在第一操作中，将空的双腔骨收获腔室（N = 8）植入动物的股骨质后作为空白对照组。在第二次操作中，植入股骨形状植入一个腔内纳米骨蛋黄+ HBMP-2质粒的腔室，分别在另一个腔中（实验组，n = 8）。每次操作之间的时间间隔为3个月。通过粗略观察，组织学检查和骨形态学分析来检索和替换腔材料，以进行评估，以比较骨α-刚性能力和碱性磷酸酶。结果：手术三个月后，纳米骨腻子+ HBMP-2质粒在腔室的一个腔内，内部有硬灰色和白色组织，而预先安装的空腔填充有软褐色组织。光学显微镜显示填充材料周围的新产生的骨组织，但只有空洞的纤维组织。纳米骨脂肪+ HBMP-2质粒组的成骨发生和碱性磷酸酶显着高于空白对照组（P <0.05）。结论：可重复使用的双腔骨收获室可用于观察HBMP-2基因活化纳米骨腻子的成骨潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号