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首页> 外文期刊>Scientia horticulturae >Identification of an up-accumulated polyamine oxidase 2 in pollen of self-incompatible 'Wuzishatangju' mandarin using comparative proteomic analysis
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Identification of an up-accumulated polyamine oxidase 2 in pollen of self-incompatible 'Wuzishatangju' mandarin using comparative proteomic analysis

机译：使用比较蛋白质组学分析鉴定自我不相容的“武出堂”普通话的花粉中累积多胺氧化酶2

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Self-incompatibility (SI) is one of the main factors causing seedless fruits in Citrus. 'Wuzishatangju' (Citrus reticulata Blanco) is a natural bud sport from a self-compatible and seedy cultivar 'Shatangju'. Our previous study showed that gametophytic SI caused seedlessness of 'Wuzishatangju'. However, it is not clear what factors are responsible for its SI response. In this study, pollen tube growth kinetics was observed in the pistils of 'Wuzishatangju' using pollination combination of 'Wuzishatangju' (female) x 'Wuzishatangju' (male) and 'Wuzishatangju' (female) x 'Shatangju' (male). The protein profiles of pollen between 'Wuzishatangju' and 'Shatangju' were compared by quantitative proteomics analysis based on tandem mass tag technology. Pollen tube from self-pollination of 'Wuzishatangju' vigorously germinated in stigma while stopped elongating at the upper site of the style. Pollen from 'Shatangju' germinated and pollen tube reached the base of style, suggesting that the mutation plays a role in pollen of 'Wuzishatangju'. Proteomic analysis indicated that a polyamine oxidase 2 (CrPAO2) was up-accumulated in pollen of 'Wuzishatangju' and catalyzed the spermine and spermidine oxidation leading to produce H2O2. The potential roles of CrPAO2 causing SI reaction in 'Wuzishatangju' were discussed.
机译：自我不相容性（Si）是导致柑橘中无籽果实的主要因素之一。 '武出堂朱'（柑橘类康塔塔布兰科）是一种自兼容和种子品种“沙塘州”的天然芽运动。我们以前的研究表明，杂草型SI引起了“武出堂”的无籽。但是，目前尚不清楚哪些因素对其SI反应负责。在这项研究中，在“武出堂”（女性）X'UBZishatangju'（Maly）和'Wuzishatangju'（Mevaly）X'Anatangju'（Male）的授粉组合，观察了花粉管生长动力学。基于串联标签技术的定量蛋白质组学分析，比较了“武出堂”和“沙塘州”之间花粉的蛋白质谱。从自我授粉的人粉碎的花粉管在耻辱中剧烈发芽，同时停止在风格的上部延伸。从“沙堂州”发芽和花粉管的花粉达到了风格的基础，这表明突变在“武出堂”的花粉中起着作用。蛋白质组学分析表明，聚胺氧化酶2（CRPAO2）在“武醉堂州”的花粉中累积，催化了前导氧化的精胺和硫胺胺氧化，导致生产H2O2。讨论了CRPAO2在'Wuzishatangju'中引起Si反应的潜在作用。

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