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首页> 外文期刊>Scientia horticulturae >Constitutive expression of a blueberry FLOWERING LOCUS T gene hastens petunia plant flowering
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Constitutive expression of a blueberry FLOWERING LOCUS T gene hastens petunia plant flowering

机译：蓝莓开花基因座T基因的组成型表达急促喇叭花植物开花

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Modulating the expression of FLOWERING LOCUS T (FT) is a powerful approach to modify plant flowering time. In this study, a blueberry (Vaccinium coiymbosum) FT (VcFT), which showed a high identity (90%) to the petunia FT gene, was used to produce early flowering petunia plants. The VcFT driven by a constitutive CaMV 35S promoter was transferred into petunia (Petunia x hybrid) plants using Agrobacterium tumefaciens-mediated transformation. Constitutive expression of VcFT (VcFT-CX) resulted in early flowering in both T-0 and T-1 transgenic plants. Compared to the controls of non-transgenic and transgenic plants that contain only the nptII selectable marker, VcFT-CX transgenic T, plants flowered 10-14 days earlier. Although the VcFT-CX transgenic T-1 plants were shorter than both controls at the start of bloom, all plants showed no significant difference in plant height at the time of seed maturity. The results demonstrated that constitutive expression of a FT-LIKE gene is highly effective in accelerating flowering for petunia plants.
机译：调制开花基因座T（FT）的表达是修改植物开花时间的强大方法。在该研究中，使用培养到Petunia FT基因的高度同一性（90％）的蓝莓（疫苗Coiymbosum）ft（Vcfft），用于生产早期开花的喇叭花植物。由组成型CAMV 35S启动子驱动的VCFT转移到Petonia（Petonia X杂交）植物中使用土壤杆菌介导的转化转移到Petonia（Petonia X杂交）植物中。 VCFT（VCFT-CX）的组成型表达导致T-0和T-1转基因植物中的早期开花。与仅含有NPTII选择标记的非转基因和转基因植物的对照相比，VCFT-CX转基因T，植物在前面为10-14天开花。虽然VCFT-CX转基因T-1植物比盛开的开始短于对照，但所有植物在种子成熟时植物高度没有显着差异。结果表明，FT样基因的组成型表达在加速Petonia植物的开花方面是高效的。

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