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首页> 外文期刊>Scientia horticulturae >Expression analysis of sorbitol transporters in pear tissues reveals that PbSOT6/20 is associated with sorbitol accumulation in pear fruits
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Expression analysis of sorbitol transporters in pear tissues reveals that PbSOT6/20 is associated with sorbitol accumulation in pear fruits

机译：梨子组织中山梨糖醇转运蛋白的表达分析表明，PBSOT6 / 20与梨果实中的山梨糖醇积累有关

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摘要

Sorbitol is a primary substrate that is translocated from its source to its sink in pear and apple by sorbitol transporters (SOTs). However, little is known about the expression profiles of SOTs in tree tissues, and their association with sorbitol accumulation. In this study, 64 SOT genes were isolated from the sequenced genomes of six rosaceae trees, which clustered phylogenetically into four groups. Groups I and III contained genes from pome trees, group II those from drupe trees, and group IV those from pome, drupe, and berry trees. A gene duplication analyses showed that the 24 SOT genes of pear arose from tandem, proximal, and dispersed duplications, which differs from their evolution in the other rosaceae fruit trees. A qRT-PCR analysis showed that the PbSOT6/20 gene in group IV is most strongly expressed in the leaf, branch, flower, fruit, and seed of pear. The expression pattern of PbSOT6/20 correlates more strongly with the pattern of sorbitol accumulation in the pear fruits than do those of other SOT genes. A low concentration (100 mg/g) of exogenous sorbitol induced the expression of PbSOT6/20 in the pear fruits, but not in the leaves. These results suggest that PbSOT6/20 is associated with sorbitol accumulation in pear fruits and plays important role in sorbitol translocation from its source to its sink. This study provides new insights into sorbitol translocation mediated by the SOT genes in pear.
机译：山梨糖醇是山梨糖醇运输扣（Sots）从其源代氏源转移到梨和苹果的源极底物。然而，对于树组织中的SOTS表达谱，及其与山梨糖醇累积的关系很少。在本研究中，从六种葡萄糖树的测序基因组中分离了64个SOT基因，该树木聚集为4组。 I和III群体含有来自污染树的墨水树的基因，以及来自Pome，Drupe和Berry树的IV组。基因重复分析表明，梨的24个SOT基因从串联，近端和分散的重复产生，这与他们的演变不同于其他鼠尾草果树。 QRT-PCR分析表明，IV组的PBSOT6 / 20基因在叶子，分支，花，果实和种子中最强烈地表达。 PBSOT6 / 20的表达模式与梨果实中山梨糖醇积累的模式相比，比其他SOT基因的模式更强烈地相关。低浓度（100mg / g）的外源山梨糖醇诱导梨果实中pbsot6 / 20的表达，但不在叶子中表达。这些结果表明，PBSOT6 / 20与梨果实中的山梨糖醇积累相关，并在山梨糖醇易位从其源到水槽中起重要作用。本研究为梨中SOT基因介导的山梨糖醇易位提供了新的见解。

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  • 来源
    《Scientia horticulturae》 |2019年第2019期|共7页
  • 作者

    Yu Cai-Yun; Cheng Hai-Yan; Cheng Rui; Qi Kai-Jie; Gu Chao; Zhang Shao-Ling;

  • 作者单位

    Nanjing Agr Univ State Key Lab Crop Genet &

    Germplasm Enhancement Ctr Pear Engn Technol Res Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ State Key Lab Crop Genet &

    Germplasm Enhancement Ctr Pear Engn Technol Res Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ State Key Lab Crop Genet &

    Germplasm Enhancement Ctr Pear Engn Technol Res Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ State Key Lab Crop Genet &

    Germplasm Enhancement Ctr Pear Engn Technol Res Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ State Key Lab Crop Genet &

    Germplasm Enhancement Ctr Pear Engn Technol Res Nanjing 210095 Jiangsu Peoples R China;

    Nanjing Agr Univ State Key Lab Crop Genet &

    Germplasm Enhancement Ctr Pear Engn Technol Res Nanjing 210095 Jiangsu Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 园艺;
  • 关键词

    Pyrus bretschneideri; Sorbitol; Sorbitol transporter; Gene expression; Phylogenetic analysis;

    机译：Pyrus Bretschneideri;山梨糖醇;山梨糖醇转运蛋白;基因表达;系统发育分析;

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