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首页> 外文期刊>Scientia horticulturae >Genetic transformation of the 'W Murcott' tangor: comparison between different techniques
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Genetic transformation of the 'W Murcott' tangor: comparison between different techniques

机译：“W Murcott”纠葛的遗传转化：不同技术的比较

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摘要

Genetic transformation of citrus has been mainly conducted via Agrobacterium tumefaciens using juvenile tissues of the epicotyl segments obtained from the in vitro germination of seeds. Transformation of the stem segments obtained from the mature material is an alternative to overcome or reduce the juvenile phase reported in perennial species such as citrus. However, some citrus species, including mandarins, are recalcitrant to these methods or have low genetic transformation efficiency. Thus, the alternative is the use of juvenile explants with high regenerative potential, such as cell suspensions obtained from citrus embryogenic callus or direct incorporation of DNA into protoplasts using polyethylene glycol (PEG). The present study aimed to compare these four genetic modification techniques for 'W Murcott', one of the main fresh market mandarin cultivars in the United States. The transgenic plants were obtained by the different techniques using a vector containing the EGFP gene under the control of the CaMV35S promoter. The genetic transformation efficiency was low, utilizing either juvenile epicotyl or mature stem segments, with an average of 1.23% and 0.33%, respectively. The genetic transformation efficiency using cell suspensions and cell suspension-derived protoplast explants were higher, with an average of 29% and 11%, respectively. Our results reveal the importance of utilizing cell suspension derived cultures for the efficient transformation of 'W Murcott'.
机译：柑橘的遗传转化主要是通过从种子的体外萌发中获得的外壳段的少年组织来进行胰蛋白酶。从成熟材料中获得的干段的转化是克服或减少在柑橘类常年物种中报告的幼年相的替代方案。然而，一些柑橘类别（包括普通酸）的柑橘类物种对这些方法顽固或具有低遗传转化效率。因此，替代方案是使用具有高再生电位的幼年外植体，例如从柑橘胚胎愈伤组织获得的细胞悬浮液或使用聚乙二醇（PEG）将DNA直接掺入原生质体中。本研究旨在比较美国主要的新鲜市场普通话制品种“W Murcott”的这四种遗传修改技术。通过在CAMV35S启动子的控制下，通过使用含有EGFP基因的载体的不同技术获得转基因植物。遗传转化效率低，利用幼年喹啉基或成熟干段，平均分别为1.23％和0.33％。使用细胞悬浮液和细胞悬浮衍生的原生质体外植体的遗传转化效率分别较高，平均分别为29％和11％。我们的结果揭示了利用细胞悬浮液衍生培养的重要性，以实现“W Murcott”的有效转变。

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