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Optimization of enzyme assisted extraction of lycopene from industrial tomato waste

机译：从工业番茄废物中辅助番茄红素的优化辅助促进番茄红素

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摘要

The present study aimed to (i) select the most suitable solvent system for extraction of lycopene from industrial tomato waste; (ii) apply enzymatic pretreatment before extraction to improve lycopene recovery; and (iii) to optimize the process conditions for the selected enzyme and solvent combination using response surface methodology. Lycopene-rich oleoresins obtained through pretreatment of waste by a combination of cellulolytic and pectinolytic enzymes followed by ethyl acetate extraction had the highest phenolic compound concentration and improved antioxidant properties, as well as the highest lycopene recovery and one of the highest red color intensity. Optimized conditions were chosen to be as follows: enzymatic reaction temperature = 40 degrees C, enzymatic reaction time = 5 h, enzyme:substrate ratio = 0.2 ml/g, solvent:substrate ratio = 5 mug, extraction time = 1 h, enzyme:enzyme ratio = 1. Oleoresin with a concentration of 11.5 mg lycopene/g was obtained at the optimum conditions. Tomato paste production waste could be valorized to produce lycopene, a valuable ingredient for food and nutraceutical industries, by combined enzymatic and solvent extraction of the waste.
机译：目前的研究旨在（i）选择最合适的溶剂系统，用于从工业番茄废物中提取番茄红素; （ii）在提取前施用酶预处理以改善番茄红素恢复; （iii）使用响应表面方法优化所选酶和溶剂组合的方法条件。通过通过纤维素分解和果胶酶的组合进行废物而获得的富含番茄红素的油树脂，然后通过乙酸乙酯萃取，具有最高的酚类化合物浓度和改善的抗氧化性能，以及最高的番茄红素回收和最高的红颜色强度之一。选择优化的条件如下：酶反应温度= 40℃，酶反应时间= 5h，酶：基板比= 0.2ml / g，溶剂：基板比= 5杯，提取时间= 1小时，酶：酶比= 1.在最佳条件下获得浓度为11.5mg番茄红素/ g的油树脂。通过组合酶促和溶剂萃取废物，可以储存番茄酱生产废物以生产番茄红素，为食品和营养营养行业提供有价值的成分。

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