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首页> 外文期刊>Spanish Journal of Agricultural Research >Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)
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Mature seeds for in vitro sanitation of the Grapevine leafroll associated virus (GLRaV-1 and GLRaV-3) from grape (Vitis vinifera L.)

机译：从葡萄（葡萄vinifera L.）的葡萄叶片相关病毒（Glrav-1和Glrav-3）的体外卫生成熟种子

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The conservation of old grapevine varieties is important since they are adapted to specific climate conditions and may carry genes interesting to breeders. As virus infection is common in grapevine varieties, the use of virus free materials is of great importance. In this work, we used somatic embryogenesis for the sanitation of GLRaV-1 and GLRaV-3 viruses that were found after analyzing the putative presence of the five most common, economically important grape viruses by real-time multiplex RT-PCR in the old cultivar "Grumet Negre". Unopened and opened inflorescences, fecundated ovaries, and, also, mature seeds were used as starting explants. Explants were cultured on plates with two embryogenesis induction media (Nitsch & McCown Woody plant medium) that contained the growth regulator thidiazuron and differed in their salt and vitamin compositions. One half of each kind of explant was cut prior to being cultured. After five months of culture, embryos had only developed from seeds that were cut previous to sowing. To the best of our knowledge, this is the first time that mature seeds have been used for inducing embryogenesis in grape. A total of 42% of the embryos transferred to tubes for germination regenerated into normal plantlets. The absence of both the GLRaV-1 and GLRaV-3 viruses in all regenerated plants was confirmed by real-time uniplex RT-PCR. So, this protocol can be used for sanitation and also for micropropagation.
机译：旧葡萄种品种的保护是重要的，因为它们适应特定的气候条件，并且可能对育种者进行有趣的基因。随着病毒感染在葡萄种品种中常见，使用病毒自由材料具有重要意义。在这项工作中，我们使用了通过在旧品种中的实时多重RT-PCR分析五种最常见的经济上重要的葡萄病毒之后发现的Glrav-1和GLRAV-3病毒的卫生植物。 “格兰特纳格雷”。未开封和开放的花序，繁殖的卵巢，以及成熟的种子被用作起始外植体。将外植体培养有两个胚胎发生介质（NITCH＆amp; McCown Woody植物培养基）的平板培养，其含有生长调节剂Thidiazuron，其盐和维生素组合物不同。在被培养之前切割每种外植体的一半。经过五个月的培养，胚胎只有从播种之前的种子制造的。据我们所知，这是第一次成熟种子已被用于诱导葡萄中的胚胎发生。总共42％的胚胎转移到用于再生成正常植物的萌发的管。通过实时的单机RT-PCR确认所有再生植物中的GLRAV-1和GLRAV-3病毒的情况。因此，该协议可用于卫生，也可以用于微荡探。

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